C5 ELISA 试剂盒
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- 抗原 See all C5 ELISA试剂盒
- C5 (Complement Component 5 (C5))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Complement Component 5 (C5). No significant cross-reactivity or interference between Complement Component 5 (C5) and analogues was observed.
- 灵敏度
- 0.073 ng/mL
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Complement Component 5 (C5) ELISA Kit
C5 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Complement Component 5 (C5) ELISA KitC5 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5 (C5) ELISA KitC5 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5 (C5) ELISA KitC5 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5 (C5) ELISA KitC5 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Component 5 (C5) ELISA KitWide Range C5 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Complement Component 5 (C5) ELISA KitC5 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum
Complement Component 5 (C5) ELISA KitC5 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Complement Component 5 (C5) ELISA KitC5 适用: 猴 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5 (C5) ELISA KitSmall Sample C5 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 5 (C5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 5 (C5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 5 (C5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 5 (C5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 5 (C5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 5 (C5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all C5 ELISA试剂盒
- C5 (Complement Component 5 (C5))
- 别名
- Complement Component 5 (C5 产品)
- 别名
- C5 ELISA Kit, LOC565774 ELISA Kit, C5a ELISA Kit, C5b ELISA Kit, CPAMD4 ELISA Kit, Hc ELISA Kit, RGD1561905 ELISA Kit, He ELISA Kit, complement C5 ELISA Kit, complement component 5 ELISA Kit, hemolytic complement ELISA Kit, C5 ELISA Kit, c5 ELISA Kit, Hc ELISA Kit
- 背景
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Gene Name: Complement Component 5
Gene Aliases: CPAMD4, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
- 途径
- Complement System, Carbohydrate Homeostasis
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