ASK1 ELISA 试剂盒
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- 抗原 See all ASK1 (MAP3K5) ELISA试剂盒
- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Apoptosis Signal Regulating Kinase 1 (ASK1). No significant cross-reactivity or interference between Apoptosis Signal Regulating Kinase 1 (ASK1) and analogues was observed.
- 灵敏度
- 0.119 ng/mL
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Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) ELISA Kit
MAP3K5 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) ELISA KitMAP3K5 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) ELISA KitMAP3K5 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptosis Signal Regulating Kinase 1 (ASK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptosis Signal Regulating Kinase 1 (ASK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptosis Signal Regulating Kinase 1 (ASK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptosis Signal Regulating Kinase 1 (ASK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptosis Signal Regulating Kinase 1 (ASK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptosis Signal Regulating Kinase 1 (ASK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ASK1 (MAP3K5) ELISA试剂盒
- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
- 别名
- Apoptosis Signal Regulating Kinase 1 (MAP3K5 产品)
- 别名
- ASK1 ELISA Kit, MAPKKK5 ELISA Kit, MEKK5 ELISA Kit, mitogen-activated protein kinase kinase kinase 5 ELISA Kit, MUD21.11 ELISA Kit, MUD21_11 ELISA Kit, 7420452D20Rik ELISA Kit, ASK ELISA Kit, Mekk5 ELISA Kit, Ask1 ELISA Kit, RGD1306565 ELISA Kit, mitogen-activated protein kinase kinase kinase 5 ELISA Kit, MAP3K5 ELISA Kit, MAPKKK5 ELISA Kit, Map3k5 ELISA Kit, map3k5 ELISA Kit
- 背景
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Gene Name: Apoptosis Signal Regulating Kinase 1
Gene Aliases: MAP3-K5, MAP3K5, ASK1, MAPKKK5, MEKK5, Mitogen-Activated Protein Kinase Kinase Kinase 5, MAPK/ERK kinase kinase 5
- 基因ID
- 4217
- UniProt
- Q99683
- 途径
- MAPK Pathway, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
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