AIF CLIA Kit
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北京 101111
Quick Overview for AIF CLIA Kit (ABIN5651955)
抗原
See all AIF (AIFM1) CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.25 pg/mL
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Apoptosis Inducing Factor (AIF). No significant cross-reactivity or interference between Apoptosis Inducing Factor (AIF) and analogues was observed.
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灵敏度
- 1.17 pg/mL
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Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) ELISA Kit
AIFM1 适用: 人 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) ELISA KitAIFM1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) ELISA KitAIFM1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) ELISA KitAIFM1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) ELISA KitAIFM1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) ELISA KitAIFM1 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) ELISA KitAIFM1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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实验时间
- 2 - 3 h
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板类型
- Pre-coated
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实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Apoptosis Inducing Factor (AIF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apoptosis Inducing Factor (AIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apoptosis Inducing Factor (AIF) level in the sample or standard.,
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptosis Inducing Factor (AIF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptosis Inducing Factor (AIF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 4-8 months
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- AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
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别名
- Apoptosis Inducing Factor
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背景
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Gene Name: Apoptosis Inducing Factor
Gene Aliases: PDCD8, Programmed Cell Death protein 8, Apoptosis-inducing factor 1, mitochondrial
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基因ID
- 9131
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UniProt
- O95831
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途径
- Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect
抗原 See all AIF (AIFM1) CLIA Kits
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