C5A ELISA 试剂盒
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- 抗原 See all C5A (C5a) ELISA试剂盒
- C5A (C5a) (Complement Component 5a (C5a))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
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This assay has high sensitivity and excellent specificity for detection of High Sensitive Complement Component 5a (C5a).
No significant cross-reactivity or interference between High Sensitive Complement Component 5a (C5a) and analogues was observed. - 灵敏度
- 35 pg/mL
- 质量等级
- High Sensitivity
- 组件
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- Pre-coated, ready to use 96-well strip plate
- Plate sealer for 96 wells
- Standard Diluent
- Assay Diluent A
- Assay Diluent B
- Stop Solution
- Standard
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Wash Buffer (30 x concentrate)
- Instruction manual
- 试剂未包括
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- Microplate reader with 450 nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution
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Complement Component 5a (C5a) ELISA Kit
C5a 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Complement Component 5a (C5a) ELISA KitC5a 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Component 5a (C5a) ELISA KitC5a 适用: 犬 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum
Complement Component 5a (C5a) ELISA KitC5a 适用: 兔 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: Pig Colorimetric Sandwich ELISA 9.375 ng/mL - 600 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: Cow Colorimetric Sandwich ELISA 4.69 ng/mL - 300 ng/mL Plasma, Serum, Tissue Homogenate
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- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Complement Component 5a (C5a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Complement Component 5a (C5a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Complement Component 5a (C5a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Complement Component 5a (C5a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验流程
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- Prepare all reagents, samples and standards,
- Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
- Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
- Aspirate and wash 3 times,
- Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
- Aspirate and wash 5 times,
- Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
- Add 50μL Stop Solution. Read at 450nm immediately.
- Prepare all reagents, samples and standards,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Complement Component 5a (C5a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Complement Component 5a (C5a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
- 限制
- 仅限研究用
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- 注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- 注意事项
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. - 储存条件
- 4 °C
- 储存方法
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
- For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit. - For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
- 有效期
- 6 months
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- 抗原 See all C5A (C5a) ELISA试剂盒
- C5A (C5a) (Complement Component 5a (C5a))
- Abstract
- C5a 产品
- 别名
- C5a ELISA Kit, C5b ELISA Kit, CPAMD4 ELISA Kit, Hc ELISA Kit, RGD1561905 ELISA Kit, complement C5 ELISA Kit, C5 ELISA Kit
- UniProt
- P06684
- 途径
- Complement System, Carbohydrate Homeostasis
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