MGMT ELISA 试剂盒
Quick Overview for MGMT ELISA 试剂盒 (ABIN5564591)
抗原
See all MGMT ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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品牌
- AssayMax™
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Analytical Method
- Quantitative
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试剂未包括
- Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)
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O6-Methylguanine-DNA-Methyltransferase (MGMT) ELISA Kit
MGMT 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
O6-Methylguanine-DNA-Methyltransferase (MGMT) ELISA KitMGMT 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
O6-Methylguanine-DNA-Methyltransferase (MGMT) ELISA KitMGMT 适用: 大鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Tissue Homogenate
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实验时间
- 4 h
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板类型
- Pre-coated
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结果分析
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- Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
- To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
- Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.
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限制
- 仅限研究用
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注意事项
- This product is for Research Use Only and is Not For Use In Diagnostic Procedures. Prepare all reagents (working diluent buffer, wash buffer, standard, biotinylated antibody, and SP Conjugate) as instructed, prior to running the assay. Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP Conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date. 2
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储存条件
- 4 °C,-20 °C
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- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
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别名
- MGMT
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基因ID
- 4255
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UniProt
- P16455
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途径
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
抗原 See all MGMT ELISA试剂盒
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