TFEB ELISA 试剂盒
This 人 TFEB ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 TFEB. This ELISA Kit has been cited in 2+ publications.
(2 references)Quick Overview for TFEB ELISA 试剂盒 (ABIN5526704)
抗原
See all TFEB products
TFEB
(Transcription Factor EB (TFEB))
适用
人
检测方法
Colorimetric
实验类型
DNA-Binding ELISA
应用范围
ELISA
样品类型
Cell Lysate, Nuclear Extract
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原理
- Human TFEB Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical TFEB binding sequences to semi-quantitatively detect active TFEB in lysates or nuclear extracts. Only available in North America.
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Analytical Method
- Semi-Quantitative
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特异性
- The olionucleotide/antibody pair provided in this kit recognizes human TFEB in whole lysates and nuclear extracts.
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产品特性
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- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96 well plate format)
- Assay can be completed within 5 hours
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组件
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- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Absorbent paper
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared HRP-secondary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
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有效期
- 6 months
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: "Lysosomal sequestration of weak base drugs, lysosomal biogenesis, and cell cycle alteration." in: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Vol. 153, pp. 113328, (2022) (PubMed).
: "HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer." in: Autophagy, Vol. 14, Issue 3, pp. 385-403, (2019) (PubMed).
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: "Lysosomal sequestration of weak base drugs, lysosomal biogenesis, and cell cycle alteration." in: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Vol. 153, pp. 113328, (2022) (PubMed).
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- TFEB (Transcription Factor EB (TFEB))
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别名
- TFEB
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基因ID
- 7942
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UniProt
- P19484
抗原 See all TFEB products
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