HDAC2 ELISA 试剂盒
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Quick Overview for HDAC2 ELISA 试剂盒 (ABIN5526697)
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适用
检测方法
实验类型
应用范围
样品类型
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原理
- Human, Mouse and Rat Phospho-HDAC2 (Ser394) and Total HDAC2 ELISA Kit. This assay semi-quantitatively measures HDAC2 phosphorylated at Serine-394 as well as total HDAC2 in cell lysate samples.
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Analytical Method
- Semi-Quantitative
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特异性
- This ELISA kit recognizes Human, Mouse and Rat HDAC2 phosphorylated at site Serine-394 as well as total HDAC2.
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产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Histone Deacetylase 2 (HDAC2) ELISA Kit
HDAC2 适用: 人, 小鼠, 大鼠 pSer394 Colorimetric Sandwich ELISA Cell Culture Lysate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
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有效期
- 6 months
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- HDAC2 (Histone Deacetylase 2 (HDAC2))
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别名
- HDAC2
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基因ID
- 3066
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UniProt
- Q92769, P70288, F7ENH8
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途径
- Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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