IL36A/IL1F6 ELISA 试剂盒
人 IL36A/IL1F6 ELISA Kit, Colorimetric is an assay for quantification of 人 IL36A/IL1F6.
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产品编号 ABIN5525085
发货至:
中国
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Quick Overview for IL36A/IL1F6 ELISA 试剂盒 (ABIN5525085)
抗原
See all IL36A/IL1F6 (IL1F6) ELISA试剂盒
IL36A/IL1F6 (IL1F6)
(Interleukin 1 Family, Member 6 (IL1F6))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.156 ng/mL - 10 ng/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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最低检测浓度
- 0.156 ng/mL
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原理
- Human IL36A (Interleukin-36 alpha) ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with IL36A , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (The matrix components in serum/plasma will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing! When the content of other samples is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Healthy serum (n=19) 1/2 dilution ND-0.22 ng/mL Healthy plasma (EDTA, Citrate , heparin) (n=20) 1/2 dilution ND-0.24 ng/mL Human peripheral blood mononuclear cells (2 x 10^6 cells/mL) were cultured in RPMI 1640 supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate for 48 hours. The cell culture supernatants were assayed for levels of human IL-36A. undiluted ND Human peripheral blood mononuclear cells (2 x 10^6 cells/mL) were cultured in RPMI 1640 supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate, followed by stimulated with 10 μg/mL PHA for 48 hours. The cell culture supernatants were assayed for levels of human IL-36A. undiluted 0.89 ng/mL 2. KD sample validation (Detect IL36A-KD PBMC cells): Sample Type Dilution Ratio Content Wild Human peripheral blood mononuclear cells (2 x 10^6 cells/mL) were cultured in RPMI 1640 supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate, followed by stimulated with 10 μg/mL PHA for 48 hours. The cell culture supernatants were assayed for levels of human IL-36A. undiluted 0.91 ng/mL Human peripheral blood mononuclear cells (+siRNA) were cultured in RPMI 1640 supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate, followed by stimulated with 10 μg/mL PHA for 48 hours. The cell culture supernatants were assayed for levels of human IL-36A. undiluted 0.12 ng/mL Note:ND is lower than the sensitivity of the kit and was not detected 3. Verified recombinant protein: (It is a normal phenomenon that some proteins have weak detection signals or cannot be detected at all due to differences in tags, sequences or protein activities) A. E.coli-derived Human IL-36A protein Lys6-Phe158
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灵敏度
- 0.094 ng/mL
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组件
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- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Biotin-labeled Antibody
- HRP-Streptavidin Conjugate(SABC
- 100X)
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Interleukin 1 Family, Member 6 (IL1F6) ELISA Kit
IL1F6 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 1 Family, Member 6 (IL1F6) ELISA KitIL1F6 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Family, Member 6 (IL1F6) ELISA KitIL1F6 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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样本量
- 100 μL
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实验时间
- 4 h
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板类型
- Pre-coated
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实验流程
- Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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- IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))
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别名
- IL36A
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背景
- Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, Interleukin-1 family member 6, IL-1F6, IL36A, FIL1E, IL1E, IL1F6
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UniProt
- Q9UHA7
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途径
- Cancer Immune Checkpoints
抗原 See all IL36A/IL1F6 (IL1F6) ELISA试剂盒
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