PKC alpha ELISA 试剂盒
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Quick Overview for PKC alpha ELISA 试剂盒 (ABIN5521385)
抗原
See all PKC alpha (PKCa) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.313 ng/mL
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原理
- Rat PKCα (Protein Kinase Cα) ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with PKCα , no obvious cross reaction with other analogues.
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灵敏度
- 0.188 ng/mL
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组件
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- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Biotin-labeled Antibody
- HRP-Streptavidin Conjugate(SABC
- 100X)
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Protein Kinase C, alpha (PKCa) ELISA Kit
PKCa 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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样本量
- 100 μL
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实验时间
- 4 h
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板类型
- Pre-coated
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实验流程
- Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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别名
- PKCalpha
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背景
- Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA
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UniProt
- P05696
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途径
- WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原 See all PKC alpha (PKCa) ELISA试剂盒
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