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PGE2 ELISA 试剂盒

Name: PGE2 ELISA 试剂盒
SKU: ABIN5520490
Availability: OutOfStock

This 人 PGE2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 PGE2. This ELISA Kit has been cited in1 publication.

ELISA

1 image

(1 reference)

产品编号 ABIN5520490

发货至: 中国

Quick Overview for PGE2 ELISA 试剂盒 (ABIN5520490)

抗原

See all PGE2 ELISA试剂盒

PGE2 (Prostaglandin E2 (PGE2))

适用

人

检测方法

Colorimetric

实验类型

Competition ELISA

检测范围

31.25 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Plasma, Serum, Tissue Homogenate

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最低检测浓度

31.25 pg/mL

原理

For quantitative detection of PGE2 in serum, plasma, tissue homogenates.

Analytical Method

Quantitative

特异性

This assay has high sensitivity and excellent specificity for detection of PGE2. No significant cross-reactivity or interference between PGE2 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between PGE2 and all the analogues, therefore, cross reaction may still exist.

灵敏度

18.75 pg/mL

组件
- ELISA Microplate (Dismountable)
- Plate sealer for 96 wells
- Lyophilized Standard
- Sample Dilution Buffer
- Biotin-labeled Antibody (Lyophilized)
- Purified water
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate (SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
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应用备注

Optimal working dilution should be determined by the investigator.

说明

Competitive ELISA, Coated with Antigen

样本量

50 μL

板类型

Pre-coated

试剂准备
- Bring all reagents and samples to room temperature for 20 minutes before use.
- Wash Buffer: If crystals have formed in the concentrate, you can warm it with 40 °C water bath Concentrated Wash Buffer into 750 mL Wash Buffer with deionized or distilled water. Put unused solution back at 2-8 °C.
- Standards:
  1. Add 1 mL Sample Dilution Buffer into one Standard tube (labeled as zero tube), keep the tube at room temperature for 10 minutes and mix them thoroughly. Note: If the standard tube concentration higher than the range of the kit,please dilute it and labeled as zero tube.
  2. Label 7 EP tubes with 1/2, 1/4, 1/8, 1/16, 1/32, 1/64 and blank respectively. Add 0.3 mLof the Sample Dilution Buffer into each tube. Add 0.3 mLof the above Standard solution (from zero tube) into 1st tube and mix them thoroughly. Transfer 0.3 mL from 1st tube to 2nd tube and mix them thoroughly. Transfer 0.3 mL from 2nd tube to 3rd tube and mix them thoroughly, and so on. Sample Dilution Buffer was used for the blank control. Note: It is best to use Standard Solutions within 2 hours.
- Preparation of Biotin-labeled Antibody Working Solution:
  Prepare it within 1 hour before experiment.
  1. Dissolve: Add 70ul purified water into tube and mix them thoroughly, after the biotin-labeled antibody is dissolved, please store it at 2-8°C.
  2. Calculate required total volume of the working solution: 50μl /well x quantity of wells. (Allow 0.1-0.2 mLmore than the total volume.)
  3. Dilute the Biotin-detection antibody with Antibody Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 µL Biotin-labeled antibody into 99 µL Antibody Dilution Buffer.)
- Preparation of HRP-Streptavidin Conjugate (SABC) Working Solution:
  Prepare it within 30 minutes before experiment.
  1. Calculate required total volume of the working solution: 0.1ml/well x quantity of wells. (Allow 0.1-0.2 mLmore than the total volume.)
  2. Dilute the SABC with SABC Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 µL of SABC into 99 µL of SABC Dilution Buffer.)
限制

仅限研究用
储存条件

4 °C

储存方法
1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
有效期

6 months
Dai, Huang, He, Zhao, Wang, Liu, Xue, Cai, Zhan, Huang, He, Wang, Wang, Sang, Li, Han, Mao, Diao, Song, Chen, Li, Man, Li, Zhou, Liu, Zhang, Li: "Acetylation Blocks cGAS Activity and Inhibits Self-DNA-Induced Autoimmunity." in: Cell, Vol. 176, Issue 6, pp. 1447-1460.e14, (2019) (PubMed).
抗原 See all PGE2 ELISA试剂盒

PGE2 (Prostaglandin E2 (PGE2))

别名

PGE2

背景

PG-E2