PGE2 ELISA 试剂盒
(13 references)-
- 抗原 See all PGE2 ELISA试剂盒
- PGE2 (Prostaglandin E2 (PGE2))
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适用
- Various Species
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 原理
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This kit recognizes PGE2 in samples. No Significant cross-reactivity or interference between PGE2 and analogues was observed.
- 灵敏度
- 18.75 pg/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
- Featured
- Discover our best selling PGE2 ELISA Kit
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- Discover our top product PGE2 ELISA Kit
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Prostaglandin E2 (PGE2) ELISA Kit
PGE2 适用: Various Species Colorimetric Competition ELISA 2.47 pg/mL - 200 pg/mL Plasma, Serum
Prostaglandin E2 (PGE2) ELISA KitPGE2 适用: 人 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Prostaglandin E2 (PGE2) ELISA KitPGE2 适用: 大鼠 Colorimetric Competition ELISA 0.5-100 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Prostaglandin E2 (PGE2) ELISA KitPGE2 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Prostaglandin E2 (PGE2) ELISA KitPGE2 适用: 大鼠 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Prostaglandin E2 (PGE2) ELISA KitPGE2 适用: 小鼠 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Prostaglandin E2 (PGE2) ELISA KitPGE2 适用: Various Species Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Prostaglandin E2 (PGE2) ELISA KitPGE2 适用: 绵羊 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Prostaglandin E2 (PGE2) ELISA KitPGE2 适用: Pig Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Prostaglandin E2 (PGE2) ELISA KitSmall Sample PGE2 适用: Various Species Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
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- 样本量
- 50 μL
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
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- Add 50 µL standard or sample to each well. Immediately add 50 µL Biotinylated Detection Antibody to each well. Incubate for 45 min at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate to each well. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
- 试剂准备
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- Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
- Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer. Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved.
- Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 2000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 2000, 1000, 500, 250, 125, 62.5, 31.25, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 2000 pg/mL stock solution to the first tube and mix up to produce a 1000 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
- Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (50 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
- Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
- 样品制备
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- It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
- If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
- If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
- Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level PGE2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level PGE2 were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %. - 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
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- For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 6 months after receipt of the kit. The reference standard, biotinylated detection antibody, HRP conjugate, and 96-well strip plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
- For used kits: When the kit is used, the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
- 有效期
- 6 months
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: "Simple hypertrophic tonsils have more active innate immune and inflammatory responses than hypertrophic tonsils with recurrent inflammation in children." in: Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale, Vol. 49, Issue 1, pp. 35, (2020) (PubMed).
: "Corydalis saxicola Alkaloids Attenuate Cisplatin-Induced Neuropathic Pain by Reducing Loss of IENF and Blocking TRPV1 Activation." in: The American journal of Chinese medicine, Vol. 48, Issue 2, pp. 407-428, (2020) (PubMed).
: "Moringa rivae leaf extracts attenuate Complete Freund's adjuvant-induced arthritis in Wistar rats via modulation of inflammatory and oxidative stress biomarkers." in: Inflammopharmacology, Vol. 28, Issue 1, pp. 139-151, (2020) (PubMed).
: "Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kβsignaling." in: Journal of reproductive immunology, Vol. 142, pp. 103192, (2020) (PubMed).
: "Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom." in: Plants (Basel, Switzerland), Vol. 9, Issue 9, (2020) (PubMed).
: "Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus." in: Metabolic brain disease, Vol. 35, Issue 4, pp. 637-647, (2020) (PubMed).
: "Gentiopicroside protects neurons from astrocyte-mediated inflammatory injuries by inhibition of nuclear factor-κB and mitogen-activated protein kinase signaling pathways." in: Neuroreport, Vol. 29, Issue 13, pp. 1114-1120, (2019) (PubMed).
: "Role of ascitic prostaglandin E2 in diagnosis of spontaneous bacterial peritonitis and prediction of in-hospital mortality in patients with decompensated cirrhosis." in: Medicine, Vol. 98, Issue 26, pp. e16016, (2019) (PubMed).
: "The Role of Prostaglandin-Endoperoxide Synthase-2 in Chemoresistance of Non-Small Cell Lung Cancer." in: Frontiers in pharmacology, Vol. 10, pp. 836, (2019) (PubMed).
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: "Chrysin Inhibits Melanoma Tumor Metastasis via Interfering with the FOXM1/β-Catenin Signaling." in: Journal of agricultural and food chemistry, Vol. 68, Issue 35, pp. 9358-9367, (2021) (PubMed).
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- 抗原 See all PGE2 ELISA试剂盒
- PGE2 (Prostaglandin E2 (PGE2))
- 别名
- Prostaglandin E2 (PGE2 产品)
- 背景
- PG-E2, Dinoprostone
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