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SIRT1 ELISA 试剂盒

Name: SIRT1 ELISA 试剂盒
SKU: ABIN5519091
Availability: OutOfStock

SIRT1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum

ELISA

1 image

(1 reference)

产品编号 ABIN5519091

发货至: 中国

Datasheet as PDF

抗原 See all SIRT1 ELISA试剂盒

SIRT1 (Sirtuin 1 (SIRT1))
适用
4

2

2
人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.31 ng/mL - 20 ng/mL

最低检测浓度

0.31 ng/mL

应用范围

ELISA

原理

The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

样品类型

Plasma, Serum

Analytical Method

Quantitative

灵敏度

0.19 ng/mL

组件
- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
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样本量

100 µL

实验时间

1 - 4.5 h

板类型

Pre-coated

实验流程
1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.
试剂准备
1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 20 ng/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 20, 10, 5, 2.5, 1.25, 0.63, 0.31, 0 ng/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 20 ng/mL working solution to the first tube and mix up to produce a 10 ng/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
限制

仅限研究用
储存条件

4 °C/-20 °C

储存方法

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
Corbi, Conti, Komici, Manzo, Filippelli, Palazzo, Vizzari, Davinelli, Di Costanzo, Scapagnini, Ferrara, Casamassima: "Phenolic Plant Extracts Induce Sirt1 Activity and Increase Antioxidant Levels in the Rabbit's Heart and Liver." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 2731289, (2018) (PubMed).
抗原 See all SIRT1 ELISA试剂盒

SIRT1 (Sirtuin 1 (SIRT1))

别名

SIRT1(Sirtuin 1) (SIRT1 产品)

别名

sirtuin 1 ELISA Kit, AA673258 ELISA Kit, SIR2L1 ELISA Kit, Sir2 ELISA Kit, Sir2a ELISA Kit, Sir2alpha ELISA Kit, sir2l1 ELISA Kit, sirtuin ELISA Kit, SIR2 ELISA Kit, sirtuin 1 ELISA Kit, sirtuin 1 L homeolog ELISA Kit, SIRT1 ELISA Kit, SRT1 ELISA Kit, Sirt1 ELISA Kit, sirt1.L ELISA Kit

途径

MAPK Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling