KREMEN1 ELISA 试剂盒
The 小鼠 KREMEN1 ELISA Kit (ABIN5510702) is a Colorimetric ELISA Kit designed to quantify 小鼠 KREMEN1.
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产品编号 ABIN5510702
发货至:
中国
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北京 101111
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Quick Overview for KREMEN1 ELISA 试剂盒 (ABIN5510702)
抗原
See all KREMEN1 ELISA试剂盒
KREMEN1
(Kringle Containing Transmembrane Protein 1 (KREMEN1))
抗原表位
AA 20-395
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
125 pg/mL - 8000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
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最低检测浓度
- 125 pg/mL
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原理
- Sandwich Mouse Kremen-1 ELISA Kit to quantitate Mouse Kremen1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A20-G395Capture antibody and Detection antibody: a monoclonal antibody from rat, a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4%, 6.5%, 7.7%
Inter-Assay Precision (CV%): 4.5%, 7.1%, 8.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- KREMEN1 (Kringle Containing Transmembrane Protein 1 (KREMEN1))
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别名
- Kremen1
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背景
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Background: Kremen protein 1 is a protein that in humans is encoded by the KREMEN1 gene. This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.
Gene Full Name: kringle containing transmembrane protein 1
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基因ID
- 84035
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UniProt
- Q99N43
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途径
- WNT signaling
抗原 See all KREMEN1 ELISA试剂盒
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