SFRP5 ELISA 试剂盒
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Quick Overview for SFRP5 ELISA 试剂盒 (ABIN5510650)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human SFRP5/Sarp3 ELISA Kit to quantitate Human SFRP5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: E30-H317Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from sheep
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 15 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Secreted Frizzled-Related Protein 5 (SFRP5) ELISA Kit
SFRP5 适用: 人 Colorimetric Sandwich ELISA 1.56-100 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Secreted Frizzled-Related Protein 5 (SFRP5) ELISA KitSFRP5 适用: 小鼠 Colorimetric Sandwich ELISA 0.25 ng/mL - 16 ng/mL Plasma, Serum
Secreted Frizzled-Related Protein 5 (SFRP5) ELISA KitSFRP5 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Secreted Frizzled-Related Protein 5 (SFRP5) ELISA KitSFRP5 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Secreted Frizzled-Related Protein 5 (SFRP5) ELISA KitHigh Sensitivity SFRP5 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Secreted Frizzled-Related Protein 5 (SFRP5) ELISA KitWide Range SFRP5 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Secreted Frizzled-Related Protein 5 (SFRP5) ELISA KitSFRP5 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Secreted Frizzled-Related Protein 5 (SFRP5) ELISA KitSFRP5 适用: 小鼠 Colorimetric Sandwich ELISA 0.25 ng/mL - 16 ng/mL Plasma, Serum
Secreted Frizzled-Related Protein 5 (SFRP5) ELISA KitSFRP5 适用: 兔 AA 30-317 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Secreted Frizzled-Related Protein 5 (SFRP5) ELISA KitSFRP5 适用: 猴 AA 30-317 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.2%, 6.5%, 4.1%
Inter-Assay Precision (CV%): 7.1%, 8.3%, 4.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))
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别名
- SFRP5
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背景
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Background: Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.
Gene Full Name: secreted frizzled related protein 5
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基因ID
- 6425
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UniProt
- Q5T4F7
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途径
- WNT signaling
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