MIA ELISA 试剂盒
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北京 101111
Quick Overview for MIA ELISA 试剂盒 (ABIN5068340)
抗原
See all MIA ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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Analytical Method
- Quantitative
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灵敏度
- 8.46 pg/mL
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产品特性
- MIA, ELISA Kit (Human) (CD-RAP)
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组件
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- Microplate, 1x96wells
- Conjugate, 1x21ml
- Standard, 1x10ng
- Assay Diluent, 1x11ml
- Callibrator Diluent, 1x21ml
- Wash buffer Concentrate, 1x21ml
- Color Reagent A, 1x12ml
- Color Reagent B, 1x12ml
- Stop Solution, 1x6ml
- Plate Sealers, 4x adhesive strips
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Melanoma Inhibitory Activity (MIA) ELISA Kit
MIA 适用: 小鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 小鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 AA 25-131 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
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板类型
- Pre-coated
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实验流程
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Principle:
- This employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human MIA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any human MIA present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for human MIA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human MIA bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C/-20°C
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- MIA (Melanoma Inhibitory Activity (MIA))
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别名
- MIA
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背景
- MIA, also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is a secreted protein expressed in cartilaginous tissue during development and in the adult. MIA was identified based on the fact that it is highly expressed in malignant melanomas, but not in normal melanocytes. MIA has been shown to interact with fibronectin, and promotes invasion and metastasis of melanoma cells, perhaps by blocking integrin-fibronectin interactions.
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