MIA ELISA 试剂盒
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- 抗原 See all MIA ELISA试剂盒
- MIA (Melanoma Inhibitory Activity (MIA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: CD RAP, melanoma inhibitory activity, Melanoma inhibitory activity protein, Melanoma-derived growth regulatory protein, Mia, MIA_HUMAN
Background: Melanoma Inhibiting Activity(MIA), also known as cartilage-derived retinoic acid-sensitive protein(CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain(1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .
Gene Name: MIA
Production: Natural and recombinant human MIA. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: G25-131Q
- Top Product
- Discover our top product MIA ELISA Kit
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Melanoma Inhibitory Activity (MIA) ELISA Kit
MIA 适用: 小鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 AA 25-131 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. It is recommended that each human MIA standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 15.6pg/mL-1000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all MIA ELISA试剂盒
- MIA (Melanoma Inhibitory Activity (MIA))
- 别名
- MIA (MIA 产品)
- 别名
- CD-RAP ELISA Kit, Mia1 ELISA Kit, Cdrap ELISA Kit, zgc:194157 ELISA Kit, mia ELISA Kit, CDRAP ELISA Kit, Fdp ELISA Kit, MIA ELISA Kit, MIAL ELISA Kit, melanoma inhibitory activity ELISA Kit, otoraplin ELISA Kit, cilia and flagella associated protein 100 ELISA Kit, MIA ELISA Kit, Mia ELISA Kit, mia ELISA Kit, Otor ELISA Kit, CFAP100 ELISA Kit
- 基因ID
- 8190
- NCBI登录号
- NP_001189482
- UniProt
- Q16674
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