MFGE8 ELISA 试剂盒
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北京 101111
Quick Overview for MFGE8 ELISA 试剂盒 (ABIN5068339)
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See all MFGE8 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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Analytical Method
- Quantitative
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灵敏度
- 13.4 pg/mL
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产品特性
- MFG-E8, Human, ELISA Kit (BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, SED1)
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组件
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- Mfg-e8 Microplate, 1x96wells
- Mfg-e8 Standard, lyophilized, 1x40ng
- Mfg-e8 Conjugate, 1x21ml
- Assay Diluent r, 1x11ml
- Calibrator Diluent r, 1x21ml
- Wash Buffer Concentrate, 25X concentrated, 1x21ml
- Color Reagent A, 1x12ml
- Color Reagent B, 1x12ml
- Stop Solution, 1x6ml
- Plate Sealers, 4x adhesive strips
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板类型
- Pre-coated
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实验流程
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Principle:
- This employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human MFG-E8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any human MFG-E8 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for human MFG-E8 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human MFG-E8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C/-20°C
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- MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
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别名
- MFG-E8
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背景
- MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.
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途径
- SARS-CoV-2 Protein Interactome
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