MFGE8 ELISA 试剂盒
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- 抗原 See all MFGE8 ELISA试剂盒
- MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
- 抗原表位
- AA 23-463
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MFGE8/Lactadherin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: A23-C463 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: A23-C463 - Featured
- Discover our best selling MFGE8 ELISA Kit
- Top Product
- Discover our top product MFGE8 ELISA Kit
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Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA Kit
MFGE8 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 适用: 人 Colorimetric Sandwich ELISA 781.25 pg/mL - 50000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 适用: 人 AA 24-387 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 适用: 小鼠 Colorimetric Sandwich ELISA 1-600 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 2 EGF-like domains.
Tissue Specificity: Mammary epithelial cell surfaces and spermatozoan. Isoform 2 is present in brain, heart, kidney and spleen and at low levels in lung, liver, small intestine and testis. .
- 板类型
- Pre-coated
- 实验流程
- mouse MFGE8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MFGE8 has been precoated onto 96-well plates. Standards(NSO, A23-C463) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MFGE8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MFGE8 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse MFGE8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MFGE8 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 528, Standard deviation: 22.2, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 1467, Standard deviation: 73.35, CV(%): 5
- Sample 3: n=16, Mean(pg/ml): 2375, Standard deviation: 116.4, CV(%): 4.9,
- Sample 1: n=24, Mean(pg/ml): 621, Standard deviation: 37.9, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 1623, Standard deviation: 113.6, CV(%): 7
- Sample 3: n=24, Mean(pg/ml): 2644, Standard deviation: 153.4, CV(%): 5.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all MFGE8 ELISA试剂盒
- MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
- 别名
- MFGE8 (MFGE8 产品)
- 别名
- BA46 ELISA Kit, EDIL1 ELISA Kit, HMFG ELISA Kit, HsT19888 ELISA Kit, MFG-E8 ELISA Kit, MFGM ELISA Kit, OAcGD3S ELISA Kit, SED1 ELISA Kit, SPAG10 ELISA Kit, hP47 ELISA Kit, ba46 ELISA Kit, edil1 ELISA Kit, oacgd3s ELISA Kit, MGC75581 ELISA Kit, hst19888 ELISA Kit, MGC145109 ELISA Kit, MFGE8 ELISA Kit, mfge8 ELISA Kit, zgc:154157 ELISA Kit, MGC99228 ELISA Kit, AA408458 ELISA Kit, AI325141 ELISA Kit, Mfgm ELISA Kit, P47 ELISA Kit, lactadherin ELISA Kit, AGS ELISA Kit, MFGME8 ELISA Kit, MFGMP-E8 ELISA Kit, im:6895406 ELISA Kit, mfge8l ELISA Kit, zgc:110846 ELISA Kit, milk fat globule-EGF factor 8 protein ELISA Kit, milk fat globule-EGF factor 8 protein b ELISA Kit, milk fat globule-EGF factor 8 protein L homeolog ELISA Kit, milk fat globule-EGF factor 8 protein a ELISA Kit, MFGE8 ELISA Kit, mfge8 ELISA Kit, mfge8b ELISA Kit, mfge8.L ELISA Kit, Mfge8 ELISA Kit, mfge8a ELISA Kit
- 背景
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Protein Function: Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha- v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction (By similarity). Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. .
Background: MFGE8(Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.
Synonyms: Lactadherin,MFGM,Milk fat globule-EGF factor 8,MFG-E8,SED1,Sperm surface protein SP47,MP47,Mfge8,
Full Gene Name: Lactadherin
Cellular Localisation: Membrane, Peripheral membrane protein . Secreted. - 基因ID
- 17304
- UniProt
- P21956
- 途径
- SARS-CoV-2 Protein Interactome
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