Histamine ELISA 试剂盒
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Quick Overview for Histamine ELISA 试剂盒 (ABIN5068087)
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See all Histamine (HIS) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA). This kit is intended for use in investigative biomedical research only. It is not for human clinical diagnostic use. This Histamine ELISA Kit should not be used for determining histamine levels in scombroid fish.
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Analytical Method
- Quantitative
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灵敏度
- 2.5 ng/mL
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产品特性
- Histamine (HIS) ELISA Kit
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组件
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- Wash Buffer (25X), 1x30ml
- TMB Substrate, 1x20ml
- PBS Sample Diluent, 1x1Liter
- Histamine Enzyme (HRP), 1x6ml
- Standard (0ng/ml), 1x500ul
- Standard (2.5ng/ml), 1x500ul
- Standard (5ng/ml), 1x500ul
- Standard (10ng/ml), 1x500ul
- Standard (20ng/ml), 1x500ul
- Standard (50ng/ml), 1x500ul
- Microtiter Plate, 1x96 wells
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Histamine (HIS) ELISA Kit
HIS 适用: Various Species Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 小鼠 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 人 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 兔 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 化学剂 Colorimetric Competition ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Histamine (HIS) ELISA KitHIS 适用: Various Species Colorimetric Sandwich ELISA 7.8-500 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 化学剂 Colorimetric Competition ELISA 5.0-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 小鼠 Colorimetric Competition ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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实验流程
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Principle of the Assay:
- This Histamine ELISA Kit is a competitive direct ELISA in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter. The microwells in this assay kit are pre-coated with a monoclonal antibody to histamine. The sample or standard solution is first added to the antibody coated microplate. Next, the enzyme conjugate is added and the mixture is shaken and incubated at RT for 45 minutes. During the incubation, unbound (free) histamine in the samples or standards is allowed to compete with enzyme HRP-labeled histamine (conjugate) for antibody binding sites. The plate is then washed, removing all the unbound material. The bound enzyme conjugate is detected by the addition of a one-component substrate which generates color by horseradish peroxidase. An optimal color is generated after 30 minutes. A microplate reader is then used to take an absorbance reading at 650nm.
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实验精密度
- Precision: ≤10% ,Precision: ≤10%
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- Histamine (HIS)
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物质类
- Chemical
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