FGF2 ELISA 试剂盒
Quick Overview for FGF2 ELISA 试剂盒 (ABIN5068059)
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检测方法
实验类型
应用范围
样品类型
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原理
- This Human Fibroblast Growth Factor basic (FGF-b) ELISA kit is to be used for the quantitative determination of FGF basic in human serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18kD isoform and recombinant FGF basic-146 and FGF basic-157.
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Analytical Method
- Quantitative
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灵敏度
- 7 pg/mL
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产品特性
- Fibroblast Growth Factor, Basic, Human (FGFb) ELISA Kit
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组件
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- Microtiter Plate, 1x96wells. FGFb antibody-coated wells.
- Standard 2x1vial.
- Standard Diluent, 1x25ml. Contains 0.1% sodium azide.
- FGFb (Biotin) Pab, 1x11ml. Contains 0.1% sodium azide.
- Streptavidin (HRP), 100X, 1x125ul. Contains 3.3mM thymol.
- Streptavidin-HRP Diluent, 1x25ml. Contains 3.3mM thymol.
- Wash Buffer Concentrate, 25X, 1x100ml.
- TMB, 1x25ml.
- Stop Solution, 1x25ml.
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Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA Kit
FGF2 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA KitFGF2 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA KitFGF2 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA KitFGF2 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA KitFGF2 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA KitFGF2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA KitFGF2 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA KitFGF2 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA KitFGF2 适用: 小鸡 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA KitSmall Sample FGF2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
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Principle of the Method:
- This human FGF basic kit is a solid phase sandwich ELISA Assay. A monoclonal antibody specific for human FGF basic has been coated onto the wells of the microtiter strips provided. Samples, including standards of known human FGF-b content, control specimens, and unknowns, are pipetted into these wells. During the first incubation, the FGF-b antigen binds to the immobilized (capture) antibody on one site. After washing, a biotinylated monoclonal antibody specific for FGF-b is added. During the second incubation, this antibody binds to the immobilized FGF-b captured during the first incubation. After removal of excess second antibody, Streptavidin-Peroxidase (enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of FGF-b present in the original specimen.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
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别名
- Fibroblast Growth Factor basic
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
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