PD-L1 ELISA 试剂盒
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Quick Overview for PD-L1 ELISA 试剂盒 (ABIN5026968)
抗原
See all PD-L1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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灵敏度
- 0.094 ng/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Mouse PDL-1 concentrations in serum, plasma. Detection Range: 0.156 - 10 ng/mL Sensitivity: <0.094 ng/mL
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组件
- plate, standard, diluent, antibodies
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试剂未包括
- pipettes, tubes, reader, incubator
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CD274 (PD-L1) ELISA Kit
PD-L1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitWide Range PD-L1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 AA 19-238 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Mouse PDL-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-Mouse PDL-1 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDL-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Mouse PDL-1 can be calculated using the standard curve.
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限制
- 仅限研究用
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
- Please refer to the Manual
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- PD-L1 (CD274 (PD-L1))
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别名
- PD-L1
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途径
- Cancer Immune Checkpoints
抗原 See all PD-L1 ELISA试剂盒
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