IFNA ELISA 试剂盒
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北京 101111
Quick Overview for IFNA ELISA 试剂盒 (ABIN5026961)
抗原
See all IFNA ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- This assay employs an antibody specific for Anti-human IFN-Alphacoated on a 96-well plate.
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Analytical Method
- Quantitative
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灵敏度
- 9.4 pg/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Human IFN-Alpha concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: <9.4 pg/mL
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组件
- plate, standard, diluent, antibodies
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试剂未包括
- pipettes, tubes, reader, incubator
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Interferon alpha (IFNA) ELISA Kit
IFNA 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon alpha (IFNA) ELISA KitIFNA 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Interferon alpha (IFNA) ELISA KitIFNA 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon alpha (IFNA) ELISA KitIFNA 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon alpha (IFNA) ELISA KitIFNA 适用: 马 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon alpha (IFNA) ELISA KitIFNA 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon alpha (IFNA) ELISA KitIFNA 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon alpha (IFNA) ELISA KitIFNA 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon alpha (IFNA) ELISA KitIFNA 适用: 猴 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Interferon alpha (IFNA) ELISA KitIFNA 适用: 兔 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This assay employs an antibody specific for Anti-human IFN-Alphacoated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-Alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IFN-Alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-Alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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限制
- 仅限研究用
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
- Please refer to the Manual
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- IFNA (Interferon alpha (IFNA))
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别名
- IFN Alpha
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途径
- JAK/STAT Signaling, TLR signaling, Hepatitis C, Inflammasome
抗原 See all IFNA ELISA试剂盒
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