IL-18 ELISA 试剂盒
Quick Overview for IL-18 ELISA 试剂盒 (ABIN4986936)
抗原
See all IL-18 (IL18) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 78 pg/mL
-
Analytical Method
- Quantitative
-
特异性
- Natural and recombinant Human IL-18 Ligand
-
灵敏度
- 20 pg/mL
-
试剂未包括
-
- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
-
-
Interleukin 18 (IL18) ELISA Kit
IL18 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Interleukin 18 (IL18) ELISA KitIL18 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Interleukin 18 (IL18) ELISA KitIL18 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitHigh Sensitivity IL18 适用: 人 Colorimetric Sandwich ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitSmall Sample IL18 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitSmall Sample IL18 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 小鸡 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Detection Wavelength: 450 nm
-
样本量
- 20 μL
-
实验时间
- 3 h
-
板类型
- Pre-coated
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
-
- IL-18 (IL18) (Interleukin 18 (IL18))
-
别名
- IL-18
-
背景
- Interleukin (IL) -18 is a newly discovered cytokine, structurally similar to IL-1, with profound effects on T-cell activation. Formerly called interferon (IFN) gamma inducing factor (IGIF), IL-18 is a novel cytokine that plays an important role in the T-cell-helper type 1 (Th1) response, primarily by its ability to induce IFN gamma production in T cells and natural killer (NK) cells. In terms of structure, IL-18 and IL-1 beta share primary amino acid sequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL-18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). Therefore inhibitors of ICE activity may limit the biologic activity of IL-18 and may be useful as Th1 immunosuppressive agents. The activity of the mature IL-18 is closely related to that of IL-1. IL-18 induces gene expression and synthesis of tumor necrosis factor (TNF), IL-1, Fas Ligand, and several chemokines. IL-18 induces the differentiation of Th1 or Th2 cells depending upon cytokine milieu and genetic background. The activity of IL-18 is via an IL-18 receptor (IL-18R) complex. This complex is made up of a binding chain termed IL-18R?, a member of the IL-1 receptor family previously identified as the IL-1 receptor - related protein (IL-1Rrp), and a signaling chain, also a member of the IL-1R family. The IL-18R complex recruits the IL-1R - activating kinase (IRAK) and TNF-R - associated factor -6 (TRAF-6) with subsequent activation of NF kappa B. Thus based on primary structure, three-dimensional structure, receptor family, signal transduction pathways and biological effects, IL-18 appears to be a new member of the IL-1 family. Similar to IL-1, IL-18 participates in both innate and acquired immunity. Constitutive IL-18 expression is detected from many different cells, including macrophages, keratinocytes, and osteoblasts. Human keratinocytes have been found to be the major producers of IL-18 in unprocessed form. An important role of IL-18 has been shown in clinical implications, in the role of IL-18 modulation in tumours, infections, and autoimmune and inflammatory diseases. Elevated serum levels of IL-18 have been shown in patients with acute graft-versus-host disease after bone marrow transplantation. Highly elevated IL-18 bioactivity with highly elevated serum IL-18 levels are measured in hematological malignancies as well as in sepsis. Increased concentrations of plasma IL-18 are found in patients with hepatic dysfunction after hepatectomy and in inflammatory liver diseases. Elevations of IL-18 levels accompany autoimmune disorders like systemic lupus erythematosus and rheumatoid arthritis. Significant levels of circulating IL-18 are found in HIV-1 infection, and other infections, in inflammatory CNS diseases, in Crohn?s disease and in pregnancy during labour and complicated pregnancies. IL-18 is regarded as a novel player in tumour immunotherapy.
-
途径
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
抗原 See all IL-18 (IL18) ELISA试剂盒
-
