Interferon gamma ELISA 试剂盒
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Quick Overview for Interferon gamma ELISA 试剂盒 (ABIN4986919)
抗原
See all Interferon gamma (IFNG) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.02 ng/mL
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant Mouse IFN-γ Ligand
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灵敏度
- 7 pg/mL
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试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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Interferon gamma (IFNG) ELISA Kit
IFNG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鸡 Colorimetric Competition ELISA 3.125 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鼠 AA 23-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum
Interferon gamma (IFNG) IQ-ELISA KitIFNG 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: Pig Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Tissue Homogenate, Urine
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应用备注
- Detection Wavelength: 450 nm
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样本量
- 20 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Interferon gamma (IFNG)
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别名
- IFN-gamma
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背景
- Interferon gamma (IFN-γ) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA (1). Produced by Tlymphocytes and natural killer (NK) cells, IFN-γ ?is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-γ?is reported to be active only on human and non-human primate cells (5). The biochemistry and biological activities of the interferons have been extensively reviewed (2-9). Mouse IFN-γ encodes a 155 amino acid (aa) residue precursor protein with a hydrophobic signal peptide that is cleaved to generate the 133 aa residue mature protein (10). In solution, IFN-γ has been shown to exist as a non-covalently associated homodimer with topological similarity to IL-10. Mouse IFN-γ shows approximately 40 % aa sequence identity to human IFN-γ and there is no cross-reactivity across species (11,12). A receptor for IFN-γ ?has been identified and its gene localized to chromosome 6 (13,14) Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-γ?(15-18). Functionally, IFN-γ ?produces a variety of effects. Produced by CD8+, NK, gd,and TH1 T helper cells, IFN-γ ?has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes (19-22) he antiprotozoal activity of IFN-γ ?against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-γ ?(23).The immunomodulatory effects of IFN-γ? are extensive and diverse. In monocyte/macrophages, the activities of IFN-γ?include: increasing the expression of class I and II MHC antigens, increasing the production of IL-1, platelet-activating factor, H2O2, and pterin, protection of monocytes against LAK cell-mediated lysis, downregulation of IL-8 mRNA expression that is upregulated by IL-2, and, with lipopolysaccharide, induction of NO production.Finally, IFN-γ?has been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells.
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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