Cytochrome C ELISA 试剂盒
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Quick Overview for Cytochrome C ELISA 试剂盒 (ABIN4986817)
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See all Cytochrome C (CYCS) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 39 pg/mL
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant Human Cytochrome C Ligand
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灵敏度
- 10 pg/mL
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试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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Cytochrome C, Somatic (CYCS) ELISA Kit
CYCS 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Cytochrome C, Somatic (CYCS) ELISA KitCYCS 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cytochrome C, Somatic (CYCS) ELISA KitCYCS 适用: 小鼠 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cytochrome C, Somatic (CYCS) ELISA KitCYCS 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cytochrome C, Somatic (CYCS) ELISA KitCYCS 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Cytochrome C, Somatic (CYCS) ELISA KitCYCS 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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应用备注
- Detection Wavelength: 450 nm
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样本量
- 20 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
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别名
- Cytochrome C
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背景
- Human somatic cytochrome c is a 15 kDa, 104 amino acid (aa) polypeptide that participates in both oxidative phosphorylation and apoptosis (1). It exhibits 91 % aa identity with mouse/rat cytochrome c (1-3). The molecule is initially synthesized in the cytoplasm as an extended, 12 kDa apoprotein. This molecule is subsequently transported across the outer mitochondrial membrane into the intermitochondrial space via a receptor-independent process. Here, it associates with an inner mitochondrial membrane enzyme called CCHL (cytochrome c heme lyase) which covalently attaches one heme molecule to the N-terminus, forming functional cytochrome c (4-6). This covalent attachment causes cytochrome c to undergo a conformational change to a globular molecule effectively trapping cytochrome c in the intermitochrondrial space.Within the intermitochondrial space, cytochrome c is actively involved in the oxidative phosphorylation pathway. It transports electrons from the cytochrome c reductase complex to the cytochrome c oxidase complex (7-9). This transports excess electrons along the respiratory pathway and generates ATP for energy-dependent processes. Alternatively, and in response to apoptotic signals, cytochrome c can be released from mitochondria into the cytosol. Here, it activates an apoptotic program via one of many possible caspase-driven cascades (8, 10-12). The events which trigger an apoptotic signal (such as growth factor withdrawal) are not well understood. The result of such a signal, however, has been suggested to be a translocation of cytosolic Bax and/or Bad to the outer mitochondrial membrane where they overcome a Bcl-2-mediated stabilization (13-15). Bax-mediated destabilization may take the form of channel creation or mitochondrial swelling, resulting in cytochrome c release (15-18). Whatever the mechanism, released cytochrome c participates in the formation of a cytosolic complex which is composed of 15 kDa cytochrome c, 130 kDa Apaf-1 (apoptotic protease activating factor-1), dATP, and 46 kDa Apaf-3/caspase-9 (15, 18, 19). Within this complex, Apaf-3/caspase-9 is activated, leading to the downstream activation of caspases-3,-7 and -9, followed by additional caspases that ultimately lead to cellular apoptosis (10, 18, 20,21).
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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