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GSK3 alpha ELISA 试剂盒

GSK3a 适用: 人, 大鼠, 小鼠 pSer21 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
产品编号 ABIN4886114
发货至: 中国
  • 抗原 See all GSK3 alpha (GSK3a) ELISA试剂盒
    GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
    抗原表位
    pSer21
    适用
    • 6
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 大鼠, 小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human/Mouse/Rat Phospho-GSK3a (S21) ELISA Kit. This assay semi-quantitatively measures phophorylated GSK3a (Ser21) in lysate samples.
    样品类型
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    特异性
    The antibody pair provided in this kit recognizes human Phospho-GSK3 alpha (pSer21).
    产品特性
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all GSK3 alpha (GSK3a) ELISA试剂盒
    GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
    别名
    GSK3a (GSK3a 产品)
    别名
    2700086H06Rik ELISA Kit, GSK3A ELISA Kit, GSK-3[a] ELISA Kit, GSK-3[b] ELISA Kit, GSK3 ELISA Kit, gsk3a ELISA Kit, xgsk3 ELISA Kit, glycogen synthase kinase 3 alpha ELISA Kit, glycogen synthase kinase 3 alpha b ELISA Kit, glycogen synthase kinase 3 alpha S homeolog ELISA Kit, GSK3A ELISA Kit, Gsk3a ELISA Kit, gsk3a ELISA Kit, gsk3ab ELISA Kit, gsk3a.S ELISA Kit, Bm1_31380 ELISA Kit
    基因ID
    2931
    UniProt
    P49840
    途径
    PI3K-Akt Signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect
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