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FGFR1 ELISA 试剂盒

FGFR1 适用: 人 pTyr Colorimetric Sandwich ELISA Cell Culture Lysate
产品编号 ABIN4886099
发货至: 中国
  • 抗原 See all FGFR1 ELISA试剂盒
    FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))
    抗原表位
    pTyr
    适用
    • 7
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Phosphotyrosine FGFR1 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine FGFR1 in lysate samples.
    样品类型
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    特异性
    This ELISA kit recognizes human FGFR1 phosphotyrosine.
    产品特性
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all FGFR1 ELISA试剂盒
    FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))
    别名
    FGFR1 (FGFR1 产品)
    别名
    FGFR ELISA Kit, fgfr ELISA Kit, X1FGFR ELISA Kit, XFGFR-1 ELISA Kit, XFGFRA2 ELISA Kit, bfgfr ELISA Kit, cek ELISA Kit, fgfr1 ELISA Kit, flg ELISA Kit, flt-2 ELISA Kit, flt2 ELISA Kit, kal2 ELISA Kit, ogd ELISA Kit, xfgfr1 ELISA Kit, CG7223 ELISA Kit, CT22273 ELISA Kit, CT39172 ELISA Kit, DFGF-R1 ELISA Kit, DFGF-R2 ELISA Kit, DFR-1 ELISA Kit, DFR1 ELISA Kit, DFR1/DFGF-R2 ELISA Kit, DPR3 ELISA Kit, DTRK(FR1) ELISA Kit, Dfr-1 ELISA Kit, Dfr1 ELISA Kit, DmHD-38 ELISA Kit, Dmel\\CG7223 ELISA Kit, Dtk1 ELISA Kit, EMS2 ELISA Kit, FGF-R2 ELISA Kit, FGFR2 ELISA Kit, FR1 ELISA Kit, Fr1 ELISA Kit, HD-38 ELISA Kit, HTL/FGFR1 ELISA Kit, Htl ELISA Kit, Tk1 ELISA Kit, dtk1 ELISA Kit, i100 ELISA Kit, i150 ELISA Kit, i79 ELISA Kit, j372 ELISA Kit, cd331 ELISA Kit, fgfbr ELISA Kit, fgfr-1 ELISA Kit, hbgfr ELISA Kit, n-sam ELISA Kit, ach ELISA Kit, cd333 ELISA Kit, cek2 ELISA Kit, fgfr-3 ELISA Kit, hsfgfr3ex ELISA Kit, jtk4 ELISA Kit, BFGFR ELISA Kit, CD331 ELISA Kit, CEK ELISA Kit, FGFBR ELISA Kit, FGFR-1 ELISA Kit, FLG ELISA Kit, FLT-2 ELISA Kit, FLT2 ELISA Kit, HBGFR ELISA Kit, HH2 ELISA Kit, KAL2 ELISA Kit, N-SAM ELISA Kit, OGD ELISA Kit, bFGF-R-1 ELISA Kit, AW208770 ELISA Kit, Eask ELISA Kit, Fgfr-1 ELISA Kit, Flt-2 ELISA Kit, Hspy ELISA Kit, FLG-1 ELISA Kit, FGFRI ELISA Kit, x1fgfr ELISA Kit, cb231 ELISA Kit, sb:cb231 ELISA Kit, fibroblast growth factor receptor ELISA Kit, fibroblast growth factor receptor 1 S homeolog ELISA Kit, basic fibroblast growth factor receptor 1 ELISA Kit, heartless ELISA Kit, fibroblast growth factor receptor 1 ELISA Kit, fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) L homeolog ELISA Kit, Fibroblast growth factor receptor 1 ELISA Kit, fibroblast growth factor receptor 1 L homeolog ELISA Kit, fibroblast growth factor receptor 1a ELISA Kit, FGFR ELISA Kit, fgfr ELISA Kit, fgfr1.S ELISA Kit, CpipJ_CPIJ005849 ELISA Kit, htl ELISA Kit, fgfr1 ELISA Kit, fgfr3.L ELISA Kit, FGFR1 ELISA Kit, Fgfr1 ELISA Kit, fgfr1.L ELISA Kit, fgfr1a ELISA Kit
    基因ID
    2260
    UniProt
    P11362
    途径
    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, S100 Proteins
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