ERBB4 ELISA 试剂盒
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- 抗原 See all ERBB4 ELISA试剂盒
- ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
- 抗原表位
- pTyr
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phosphotyrosine ErbB4 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine ErbB4 in lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes human ErbB4 phosphotyrosine.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our top product ERBB4 ELISA Kit
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V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) ELISA Kit
ERBB4 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) ELISA KitERBB4 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) ELISA KitERBB4 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) ELISA KitERBB4 适用: 人 Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all ERBB4 ELISA试剂盒
- ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
- 别名
- ErbB4 (ERBB4 产品)
- 别名
- erbb4 ELISA Kit, si:ch211-227n20.1 ELISA Kit, Her4 ELISA Kit, c-erbB-4 ELISA Kit, HER4 ELISA Kit, p180erbB4 ELISA Kit, erb-b2 receptor tyrosine kinase 4 ELISA Kit, erb-b2 receptor tyrosine kinase 4a ELISA Kit, v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) ELISA Kit, erb-b2 receptor tyrosine kinase 4 L homeolog ELISA Kit, receptor tyrosine-protein kinase ErbB-4 ELISA Kit, ERBB4 ELISA Kit, erbb4a ELISA Kit, erbb4 ELISA Kit, erbb4.L ELISA Kit, Tsp_13582 ELISA Kit, Erbb4 ELISA Kit
- 基因ID
- 2066
- UniProt
- Q15303
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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