Angiopoietin 4 ELISA 试剂盒
Quick Overview for Angiopoietin 4 ELISA 试剂盒 (ABIN4885695)
抗原
See all Angiopoietin 4 (ANGPT4) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- Custom Mouse Angiopoietin-3 (ANGPTL1) SpeedELISA Kit.
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品牌
- SpeedELISA
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Analytical Method
- Quantitative
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产品特性
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- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Angiopoietin 4 (ANGPT4) ELISA Kit
ANGPT4 适用: 人 Colorimetric Sandwich ELISA 18.75 pg/mL - 1200 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 4 (ANGPT4) ELISA KitANGPT4 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 4 (ANGPT4) ELISA KitANGPT4 适用: 人 Colorimetric Sandwich ELISA 0.25-200 ng/mL Cell Culture Supernatant, Plasma, Serum
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. -
有效期
- 6 months
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- Angiopoietin 4 (ANGPT4)
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别名
- Angiopoietin-3
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基因ID
- 72713
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UniProt
- Q640P2
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途径
- RTK signaling
抗原 See all Angiopoietin 4 (ANGPT4) ELISA试剂盒
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