IGFBP3 ELISA 试剂盒
-
- 抗原 See all IGFBP3 ELISA试剂盒
- IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human IGFBP-3 SpeedELISA Kit.
- 品牌
- SpeedELISA
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 产品特性
-
- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling IGFBP3 ELISA Kit
- Top Product
- Discover our top product IGFBP3 ELISA Kit
-
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit
IGFBP3 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 小鸡 Colorimetric Competition ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 犬 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: Cow Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
-
- 样本量
- 100 μL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
-
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. - 有效期
- 6 months
-
- 抗原 See all IGFBP3 ELISA试剂盒
- IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
- 别名
- IGFBP-3 (IGFBP3 产品)
- 别名
- BP-53 ELISA Kit, IBP3 ELISA Kit, AI649005 ELISA Kit, IGFBP-3 ELISA Kit, IGgfbp3 ELISA Kit, IGF-BP3 ELISA Kit, zgc:91788 ELISA Kit, IGFBP3 ELISA Kit, igfbp3 ELISA Kit, insulin like growth factor binding protein 3 ELISA Kit, insulin-like growth factor binding protein 3 ELISA Kit, IGFBP3 ELISA Kit, Igfbp3 ELISA Kit, igfbp3 ELISA Kit, IGFBP-3 ELISA Kit, LOC100305016 ELISA Kit
- 基因ID
- 3486
- UniProt
- P17936
- 途径
- Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
-
