Adipsin ELISA 试剂盒
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- 抗原 See all Adipsin (CFD) ELISA试剂盒
- Adipsin (CFD) (Complement Factor D (CFD))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Adipsin (Complement Factor D) SpeedELISA Kit.
- 品牌
- SpeedELISA
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 产品特性
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- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Complement Factor D (CFD) ELISA Kit
CFD 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Factor D (CFD) ELISA KitCFD 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Complement Factor D (CFD) ELISA KitCFD 适用: 小鼠 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Factor D (CFD) ELISA KitCFD 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Complement Factor D (CFD) ELISA KitCFD 适用: 人 AA 26-253 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Complement Factor D (CFD) ELISA KitCFD 适用: 小鼠 AA 26-259 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 样本量
- 100 μL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. - 有效期
- 6 months
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- 抗原 See all Adipsin (CFD) ELISA试剂盒
- Adipsin (CFD) (Complement Factor D (CFD))
- 别名
- Adipsin (CFD 产品)
- 别名
- ADIPSIN ELISA Kit, ADN ELISA Kit, DF ELISA Kit, PFD ELISA Kit, Adn ELISA Kit, Df ELISA Kit, EVE ELISA Kit, CFD ELISA Kit, adipsin ELISA Kit, pfd ELISA Kit, cfdl ELISA Kit, wu:fb61f12 ELISA Kit, zgc:109940 ELISA Kit, 10.5 ELISA Kit, 10.9 ELISA Kit, 14.10 ELISA Kit, 20.35 ELISA Kit, CG2328 ELISA Kit, Dmel\\CG2328 ELISA Kit, E(eve) ELISA Kit, Eve ELISA Kit, F ELISA Kit, V ELISA Kit, VI ELISA Kit, eve2 ELISA Kit, even ELISA Kit, l(2)46CFg ELISA Kit, l(2)46CFh ELISA Kit, l(2)46CFj ELISA Kit, l(2)46CFp ELISA Kit, l(2)46Ce ELISA Kit, l(2)46Cg ELISA Kit, complement factor D ELISA Kit, complement factor D (adipsin) ELISA Kit, complement factor D (adipsin) L homeolog ELISA Kit, even skipped ELISA Kit, CFD ELISA Kit, Cfd ELISA Kit, cfd.L ELISA Kit, cfd ELISA Kit, eve ELISA Kit
- 基因ID
- 1675
- UniProt
- P00746
- 途径
- Complement System
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