SMAD4 ELISA 试剂盒
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- 抗原 See all SMAD4 ELISA试剂盒
- SMAD4 (SMAD Family Member 4 (SMAD4))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human SMAD4 ELISA Kit for Cell Culture Supernatants.
- 样品类型
- Cell Culture Supernatant
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human SMAD4.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling SMAD4 ELISA Kit
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- Discover our top product SMAD4 ELISA Kit
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SMAD Family Member 4 (SMAD4) ELISA Kit
SMAD4 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
SMAD Family Member 4 (SMAD4) ELISA KitSMAD4 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
SMAD Family Member 4 (SMAD4) ELISA KitSMAD4 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
SMAD Family Member 4 (SMAD4) ELISA KitSMAD4 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all SMAD4 ELISA试剂盒
- SMAD4 (SMAD Family Member 4 (SMAD4))
- 别名
- Smad4 (SMAD4 产品)
- 别名
- smad4 ELISA Kit, DPC4 ELISA Kit, JIP ELISA Kit, MADH4 ELISA Kit, MYHRS ELISA Kit, AW743858 ELISA Kit, D18Wsu70e ELISA Kit, Madh4 ELISA Kit, XSmad4alpha ELISA Kit, Xsmad4 ELISA Kit, dpc4 ELISA Kit, jip ELISA Kit, madh4 ELISA Kit, xsmad4a ELISA Kit, SMAD family member 4 ELISA Kit, Smad4 protein ELISA Kit, SMAD family member 4, gene 1 L homeolog ELISA Kit, SMAD4 ELISA Kit, smad4 ELISA Kit, Smad4 ELISA Kit, smad4.1.L ELISA Kit
- 基因ID
- 4089
- UniProt
- Q13485
- 途径
- Cell Division Cycle, Chromatin Binding, Autophagy
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