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GATA3 ELISA 试剂盒
GATA3
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
GATA3ELISA Kit详情
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GATA3
(GATA Binding Protein 3 (GATA3))
适用
All reactivities for GATA3 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human GATA-3 ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes .
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
GATA3目标详情
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GATA3
(GATA Binding Protein 3 (GATA3))
别名
GATA-3 (GATA3 产品 )
别名
HDR ELISA Kit, HDRS ELISA Kit, Gata-3 ELISA Kit, jal ELISA Kit, cb550 ELISA Kit, gta3 ELISA Kit, GATA-3 ELISA Kit, XGATA3 ELISA Kit, xgata-3 ELISA Kit, hdr ELISA Kit, xGATA-3 ELISA Kit, GATA3 ELISA Kit, GATA transcription factor 3 ELISA Kit, T4L20.260 ELISA Kit, T4L20_260 ELISA Kit, gata3 ELISA Kit, ZH-DR ELISA Kit, zgc:103531 ELISA Kit, GATA binding protein 3 ELISA Kit, GATA binding protein 3 S homeolog ELISA Kit, GATA transcription factor 3 ELISA Kit, amidase ELISA Kit, putative amidase ELISA Kit, GATA-binding protein 3 ELISA Kit, hematopoietic death receptor ELISA Kit, GATA3 ELISA Kit, Gata3 ELISA Kit, gata3 ELISA Kit, gata3.S ELISA Kit, gatA3 ELISA Kit, hdr ELISA Kit
基因ID
2625
UniProt
P23771
途径
Hormone Transport , Regulation of Hormone Metabolic Process , Tube Formation
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