APEX1 ELISA 试剂盒
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- 抗原 See all APEX1 ELISA试剂盒
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human APE1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human CD177.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Apurinic/Apyrimidinic Endonuclease 1 (APEX1) ELISA Kit
APEX1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) ELISA KitAPEX1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) ELISA KitAPEX1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all APEX1 ELISA试剂盒
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
- 别名
- APE1 (APEX1 产品)
- 别名
- apex1 ELISA Kit, MGC76280 ELISA Kit, MGC79645 ELISA Kit, MGC78928 ELISA Kit, APE ELISA Kit, APE1 ELISA Kit, APEN ELISA Kit, APEX ELISA Kit, APX ELISA Kit, HAP1 ELISA Kit, REF1 ELISA Kit, Apex ELISA Kit, REF-1 ELISA Kit, BAP1 ELISA Kit, apex1a ELISA Kit, hm:zehn1532 ELISA Kit, wu:fc13d11 ELISA Kit, zgc:66204 ELISA Kit, Ref-1 ELISA Kit, apurinic/apyrimidinic endodeoxyribonuclease 1 ELISA Kit, APEX nuclease (multifunctional DNA repair enzyme) 1 ELISA Kit, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog ELISA Kit, DNA-(apurinic or apyrimidinic site) lyase ELISA Kit, apurinic/apyrimidinic endonuclease 1 ELISA Kit, APEX1 ELISA Kit, apex1 ELISA Kit, apex1.L ELISA Kit, CNG03640 ELISA Kit, CNH01280 ELISA Kit, nth ELISA Kit, Spirs_4119 ELISA Kit, Palpr_0103 ELISA Kit, Apex1 ELISA Kit
- 基因ID
- 328
- UniProt
- P27695
- 途径
- DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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