Angiotensin I Converting Enzyme 1 ELISA 试剂盒
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- 抗原 See all Angiotensin I Converting Enzyme 1 (ACE) ELISA试剂盒
- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human ACE (CD143) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes human ACE. Other species not yet determined.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product ACE ELISA Kit
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Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA Kit
ACE 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cerebrospinal Fluid, Plasma, Serum
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 人 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 绵羊 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 豚鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 兔 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all Angiotensin I Converting Enzyme 1 (ACE) ELISA试剂盒
- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
- 别名
- ACE (ACE 产品)
- 别名
- ACE1 ELISA Kit, CD143 ELISA Kit, DCP ELISA Kit, DCP1 ELISA Kit, ICH ELISA Kit, MVCD3 ELISA Kit, AW208573 ELISA Kit, Dcp1 ELISA Kit, StsRR92 ELISA Kit, dcp ELISA Kit, ace1 ELISA Kit, dcp1 ELISA Kit, xace ELISA Kit, cd143 ELISA Kit, ACE ELISA Kit, angiotensin I converting enzyme ELISA Kit, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 ELISA Kit, angiotensin-converting enzyme ELISA Kit, angiotensin I converting enzyme (peptidyl-dipeptidase A) 3 ELISA Kit, angiotensin-converting enzyme-like ELISA Kit, ACE ELISA Kit, Ace ELISA Kit, ace ELISA Kit, CpipJ_CPIJ009106 ELISA Kit, ACE3 ELISA Kit, LOC101824864 ELISA Kit
- 基因ID
- 1636
- UniProt
- P12821
- 途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
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