PLAU ELISA 试剂盒
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Quick Overview for PLAU ELISA 试剂盒 (ABIN456751)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of human prolactin concentrations in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- 5 This assay recognizes recombinant and natural human prolactin.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
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< 0.039 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. -
产品特性
- Homo sapiens,Human,Urokinase-type plasminogen activator,U-plasminogen activator,uPA,PLAU,3.4.21.73
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Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU 适用: 马 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to prolactin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for prolactin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain prolactin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of prolactin in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- PLAU (Plasminogen Activator, Urokinase (PLAU))
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别名
- PLAU
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背景
- UPA is a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. For example, the combination (both low vs. either or both high) of uPA and its inhibitor, plasminogen activator inhibitor-1 (PAI-1), outperforms the single factors as well as other traditional prognostic factors with regard to risk group assessment for breast cancer, particularly in node-negative breast cancer. The uPA is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule. The B chain starting at Ile179 corresponds to the catalytic domain. Two forms of the A chain exist, one starting at Ser21 (the long form) and the other at Lys156 (the short form). The resulting two-chain forms have different molecular weights (MW). The B chain is common for both forms whereas the long and short A chains are unique to the high and low MW forms, respectively. The long A chain contains an EGF-like domain, which is responsible for binding of the uPA receptor (uPAR). Both high and low MW forms exist in the purified recombinant Rabbit uPA.
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途径
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
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