MMP2 ELISA 试剂盒
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Quick Overview for MMP2 ELISA 试剂盒 (ABIN455836)
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See all MMP2 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.312 ng/mL
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原理
- This immunoassay kit allows for the specific measurement of mouse matrix metalloproteinase 2/Gelatinase A, MMP-2 concentrations in cell culture supernates, serum and plasma.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural mouse MMP-2.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
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产品特性
- Mus musculus,Mouse,72 kDa type IV collagenase,72 kDa gelatinase,Gelatinase A,Matrix metalloproteinase-2,MMP-2,Mmp2,3.4.24.24
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Matrix Metalloproteinase 2 (MMP2) ELISA Kit
MMP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 马 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 大鼠 AA 30-662 Colorimetric Sandwich ELISA 625-40000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 AA 30-660 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 小鼠 AA 30-662 Colorimetric Sandwich ELISA 625-40000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique. A antibody specific for MMP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-2 present is bound by the immobilized antibody. An enzyme-linked antibody specific for MMP-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- MMP2 (Matrix Metalloproteinase 2 (MMP2))
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别名
- Mmp2
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背景
- Matrix metalloproteinases (MMPs), also called matrixins, constitute a family of zinc and calcium-dependent endopeptidases that function in the breakdown of extracellular matrix and in the processing of a variety of biological molecules. They play an important role in many normal physiological processes such as embryonic development, morphogenesis, reproduction and tissue remodeling . They also participate in many pathological processes such as arthritis, cancer and cardiovascular disease . While the amounts of newly synthesized MMPs are regulated mainly at the levels of transcription, the proteolytic activities of existing MMPs are controlled through both the activation of proenzymes or zymogens and the inhibition of active enzymes by endogenous inhibitors such as α2-macroglobulins and tissue inhibitors of metalloproteinases (TIMPs). MMP-2 (gelatinase A) is primarily expressed in mesenchymal cells (mainly fibroblasts) during development and tissue regeneration. It is highly expressed in stromal cells surrounding the invading front of metastasizing tumors and associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. It is required for the switch to the angiogenic phenotype in a tumor model and its levels are elevated in tumor endothelium and in urine of patients with a variety of cancers. Together with MMP-9 (gelatinase B), it degrades type IV collagen, the major component of basement membranes and gelatin (denatured collagen). It can also degrade other types of collagens (V, VII and X) as well as elastin and fibronectin. It processes many other molecules, modulating their functions in different pathways. Human and mouse MMP-2 are secreted as 72 kDa proenzymes of 631 and 662 amino acids, respectively. The removal of the pro domain can be initiated by membrane-type MMPs or by serine proteases such as thrombin and activated protein C. The resulting mature and active enzyme consists of a catalytic domain, which is interrupted by three contiguous fibronectin type II-like domains, and a C-terminal, hemopexin-like domain. TIMPs inhibit active MMP-2 through tight, but non-covalent binding of their N-terminal domains to the catalytic domain of MMP-2 in a 1:1 stoichiometry. In addition, TIMP-2 can bind to the proenzyme through interaction between the C-terminal domains of both proteins. 2
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途径
- Activation of Innate immune Response
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