HAVCR1 ELISA 试剂盒
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Quick Overview for HAVCR1 ELISA 试剂盒 (ABIN455655)
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See all HAVCR1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This immunoassay kit allows for the specific measurement of Mouse KIM-1 concentrations in serum,urine and plasma.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural Mouse KIM-1.
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交叉反应 (详细)
- No significant cross-reactivity or interference was observed.
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产品特性
- Mus musculus,Mouse,Hepatitis A virus cellular receptor 1 homolog,HAVcr-1,Kidney injury molecule 1,KIM-1,T cell immunoglobulin and mucin domain-containing protein 1,TIMD-1,T cell membrane protein 1,TIM-1,
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for KIM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KIM-1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for KIM-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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: "Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication." in: Clinical toxicology (Philadelphia, Pa.), Vol. 47, Issue 9, pp. 870-5, (2009) (PubMed).
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- HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
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别名
- Havcr1
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物质类
- Virus
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背景
- Kidney injury molecule-1 (KIM-1) is a type 1 transmembrane protein that is not detectable in normal kidney tissue but is expressed at high levels in rodent kidneys with dedifferentiated proximal tubule epithelial cells after ischemic or toxic injury.
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