HAVCR1 ELISA 试剂盒
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Quick Overview for HAVCR1 ELISA 试剂盒 (ABIN921116)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich Rat KIM1 ELISA Kit to quantitate Rat Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. .
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: S18-V238Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 2 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA Kit
HAVCR1 适用: 小鼠 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Tissue Homogenate, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 人 AA 21-288 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 猴 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Serum, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 犬 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.4%, 7.6%, 7.9%
Inter-Assay Precision (CV%): 4.6%, 7.9%, 8.2% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Involvement of the NF-κB signaling pathway in the renoprotective effects of isorhamnetin in a type 2 diabetic rat model." in: Biomedical reports, Vol. 4, Issue 5, pp. 628-634, (2016) (PubMed).
: "Glycyrrhetinic acid might increase the nephrotoxicity of bakuchiol by inhibiting cytochrome P450 isoenzymes." in: PeerJ, Vol. 4, pp. e2723, (2016) (PubMed).
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- HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
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别名
- HAVCR1
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物质类
- Virus
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背景
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Background: KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.
Gene Full Name: hepatitis A virus cellular receptor 1
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基因ID
- 286934
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UniProt
- O54947
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