Interferon gamma ELISA 试剂盒
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Quick Overview for Interferon gamma ELISA 试剂盒 (ABIN455496)
抗原
See all Interferon gamma (IFNG) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of mouse IFN- ? concentrations in cell culture supernates, serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural mouse IFN- ? .
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
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< 3.9 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. -
产品特性
- Mus musculus,Mouse,Interferon gamma,IFN-gamma,Ifng
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Interferon gamma (IFNG) ELISA Kit
IFNG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鸡 Colorimetric Competition ELISA 3.125 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鼠 AA 23-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum
Interferon gamma (IFNG) IQ-ELISA KitIFNG 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Tissue Homogenate, Urine
Interferon gamma (IFNG) ELISA KitIFNG 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: Pig Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 豚鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFN- γ . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IFN- γ . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IFN- γ , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a 2 change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IFN- γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Interferon gamma (IFNG)
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别名
- Ifng
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背景
- IFN- γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor. The IFN- γ monomer consists of a core of six α -helices and an extended unfolded sequence in the C-terminal region. The biologically active dimer is formed by anti-parallel inter-locking of two monomers. Full length of IFN- γ is 143 amino acids. In contrast to interferon- α and interferon- β which can be expressed by all cells, IFN- γ is secreted by T lymphocytes and NK cells only. Also known as immune interferon, IFN- γ is the only Type II interferon. It is serologically distinct from Type I interferons and it is acid-labile, while the type I variants are acid-stable. IFN- γ has antiviral, immunoregulatory, and anti-tumour properties. It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. Activation by IFN- γ is achieved by its interaction with a heterodimeric receptor consisting of IFNGR1 & IFNGR2 (interferon gamma receptors). IFN- γ binding to the receptor activates the JAK-STAT pathway. In addition, IFN- γ activates APCs and promotes Th1 differentiation by upregulating the transcription factor T-bet. IFN- γ is the hallmark cytokine of Th1 cells (Th2 cells produce IL-4). NK cells and [[CD8+ cytotoxic T cell]]s also produce IFN- γ . IFN- γ suppresses osteoclast formation by rapidly degrading the RANK adaptor protein TRAF6 in the RANK-RANKL signaling pathway, which otherwise stimulates the production of NF κ B.
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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