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SFRP2 ELISA 试剂盒

SFRP2 适用: 小鼠 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN455159
发货至: 中国
  • 抗原 See all SFRP2 ELISA试剂盒
    SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.78-50 ng/mL
    最低检测浓度
    0.78 ng/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the in vitro quantitative determination of mouse Free-T3 concentrations in cell culture supernates, serum, plasma and other biological fluids.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This assay recognizes recombinant and natural mouse Free-T3.
    交叉反应 (详细)
    No significant cross-reactivity or interference was observed.
    灵敏度
    < 0.078 pmol/L
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    产品特性
    Mus musculus,Mouse,Secreted frizzled-related protein 2,sFRP-2,Protein SDF5,Secreted apoptosis-related protein 1,SARP-1,Sfrp2,Sarp1,Sdf5
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    Discover our best selling SFRP2 ELISA Kit
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    Discover our top product SFRP2 ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to Free-T3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Free-T3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Free-T3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Free-T3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    限制
    仅限研究用
  • 储存条件
    4 °C/-20 °C
    储存方法
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • 抗原 See all SFRP2 ELISA试剂盒
    SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))
    别名
    Sfrp2 (SFRP2 产品)
    别名
    frp-2 ELISA Kit, sarp1 ELISA Kit, sdf-5 ELISA Kit, sfrp-2 ELISA Kit, SFRP2 ELISA Kit, sfrp2 ELISA Kit, FRP-2 ELISA Kit, SARP1 ELISA Kit, SDF-5 ELISA Kit, AI851596 ELISA Kit, Sdf5 ELISA Kit, hm:zeh0225 ELISA Kit, zeh0225 ELISA Kit, zgc:153618 ELISA Kit, SFRP-2 ELISA Kit, secreted frizzled-related protein 2 ELISA Kit, secreted frizzled related protein 2 ELISA Kit, secreted frizzled-related protein 2 S homeolog ELISA Kit, secreted frizzled-related protein ELISA Kit, sfrp2 ELISA Kit, sfrp2 ELISA Kit, SFRP2 ELISA Kit, sfrp2.S ELISA Kit, LOAG_01461 ELISA Kit, Sfrp2 ELISA Kit
    途径
    WNT signaling, Tube Formation, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Positive Regulation of fat Cell Differentiation
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