CCL24 ELISA 试剂盒
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Quick Overview for CCL24 ELISA 试剂盒 (ABIN455041)
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See all CCL24 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- This immunoassay kit allows for the specific measurement of mouse Eotaxin-2 (CCL24) concentrations in cell culture supernates, serum, plasma , BALF and tissue homogenates.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural mouse CCL24. The factors listed below were assayed for cross-reactivity.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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产品特性
- Mus musculus,Mouse,C-C motif chemokine 24,Eosinophil chemotactic protein 2,Eotaxin-2,Small-inducible cytokine A24,Ccl24,Scya24
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Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA Kit
CCL24 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 人 AA 27-119 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 小鼠 AA 27-119 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 人 Colorimetric Sandwich ELISA
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CCL24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL24 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for CCL24 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL24 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))
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别名
- Ccl24
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背景
- Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and Il-4. LPS and IL-4 also differentially regulate the expression of eotaxin-2 in monocytes and macrophages. Mouse eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human eotaxin-2. Functionally, eotaxin-2 is most closely related to eotaxin/CCL11 and eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin.
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途径
- Regulation of Actin Filament Polymerization
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