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CCL24 ELISA 试剂盒

CCL24 适用: 小鼠 AA 27-119 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN1672758
发货至: 中国
  • 抗原 See all CCL24 ELISA试剂盒
    CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))
    抗原表位
    AA 27-119
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6-1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL24/Eotaxin-2
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: E.coli
    Immunogen sequence: V27-V119
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: E.coli
    Immunogen sequence: V27-V119
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Tissue Specificity: Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis. .

    板类型
    Pre-coated
    实验流程
    mouse CCL24 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL24 has been precoated onto 96-well plates. Standards(E.coli, V27-V119) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL24 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL24 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CCL24 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CCL24 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 125, Standard deviation: 6, CV(%): 4.8
    • Sample 2: n=16, Mean(pg/ml): 257, Standard deviation: 10.79, CV(%): 4.2
    • Sample 3: n=16, Mean(pg/ml): 526, Standard deviation: 33.14, CV(%): 6.3,
    • Sample 1: n=24, Mean(pg/ml): 130, Standard deviation: 10.14, CV(%): 7.8
    • Sample 2: n=24, Mean(pg/ml): 249, Standard deviation: 15.94, CV(%): 6.4
    • Sample 3: n=24, Mean(pg/ml): 518, Standard deviation: 42.99, CV(%): 8.3
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all CCL24 ELISA试剂盒
    CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))
    别名
    CCL24 (CCL24 产品)
    别名
    Ckb-6 ELISA Kit, MPIF-2 ELISA Kit, MPIF2 ELISA Kit, SCYA24 ELISA Kit, CKb-6 ELISA Kit, Scya24 ELISA Kit, C-C motif chemokine ligand 24 ELISA Kit, chemokine (C-C motif) ligand 24 ELISA Kit, CCL24 ELISA Kit, Ccl24 ELISA Kit
    背景

    Protein Function: Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Binds to CCR3 (By similarity). .

    Background: Chemokine(C-C motif) ligand 24(CCL24) also known as myeloid progenitor inhibitory factor 2(MPIF-2) or eosinophil chemotactic protein 2(eotaxin-2) is a protein that in humans is encoded by the CCL24 gene.1 By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23.2 CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

    Synonyms: C-C motif chemokine 24,Eosinophil chemotactic protein 2,Eotaxin-2,Small-inducible cytokine A24,Ccl24,Scya24,

    Full Gene Name: C-C motif chemokine 24

    Cellular Localisation: Secreted.
    基因ID
    56221
    UniProt
    Q9JKC0
    途径
    Regulation of Actin Filament Polymerization
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