ENOS ELISA 试剂盒
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Quick Overview for ENOS ELISA 试剂盒 (ABIN455020)
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See all ENOS (NOS3) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- This immunoassay kit allows for the in vitro quantitative determination of Mouse Endothelial nitric oxide synthase 3, eNOS-3, concentrations in serum, tissue homogenates and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural mouse eNOS-3.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
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产品特性
- Mus musculus,Mouse,Nitric oxide synthase, endothelial,Constitutive NOS,cNOS,EC-NOS,Endothelial NOS,eNOS,NOS type III,NOSIII,Nos3,Ecnos,1.14.13.39
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Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA Kit
NOS3 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 IU/mL - 20 IU/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 适用: Cow Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 适用: 豚鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 适用: 兔 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to eNOS-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for eNOS-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain eNOS-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of eNOS-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
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别名
- Nos3
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途径
- ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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