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Angiopoietin 1 ELISA 试剂盒

ANGPT1 适用: 人 Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN414353
发货至: 中国
  • 抗原 See all Angiopoietin 1 (ANGPT1) ELISA试剂盒
    Angiopoietin 1 (ANGPT1)
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.24 ng/mL - 15 ng/mL
    最低检测浓度
    0.24 ng/mL
    应用范围
    ELISA
    原理
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ANGPT1 in Serum,Plasma,Biological Fluids
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性

    This assay has high sensitivity and excellent specificity for detection of Angiopoietin 1 (ANGPT1).
    No significant cross-reactivity or interference between Angiopoietin 1 (ANGPT1) and analogues was observed.

    交叉反应 (详细)
    No significant cross-reactivity or interference between Angiopoietin 1 (ANGPT1) and analogues was observed.
    灵敏度
    0.11 ng/mL
    组件
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
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  • 应用备注
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    说明

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    样本量
    100 μL
    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin 1 (ANGPT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin 1 (ANGPT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin 1 (ANGPT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin 1 (ANGPT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    试剂准备
    1. Bring all kit components and samples to room temperature (18-25 °C) before use.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 30 ng/mL. Firstly dilute the stock solution to 15 ng/mL and the diluted standard serves as the highest standard (15 ng/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 15 ng/mL, 7.5 ng/mL, 3.75 ng/mL, 1.88 ng/mL, 0.94 ng/mL, 0.47 ng/mL, 0.24 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
    3. Detection Reagent A and Detection Reagent B - Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute to the working concentration with Assay Diluent A and B, respectively (1:100).
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for once pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    6. Contaminated water or container for reagent preparation will influence the detection result.
    实验精密度

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin 1 (ANGPT1) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin 1 (ANGPT1) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    限制
    仅限研究用
  • 注意事项
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    注意事项
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    储存条件
    4 °C
    储存方法
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    有效期
    6 months
  • Wang, Liu, Xu, Qian, Zou, Yi, Guan, Yin: "Association between Upper-airway Surgery and Ameliorative Risk Markers of Endothelial Function in Obstructive Sleep Apnea." in: Scientific reports, Vol. 9, Issue 1, pp. 20157, (2020) (PubMed).

    Shibata, Nakai, Ozaki, Koshi, Tanaka, Morita, Maeno, Kawato: "Effects of interleukin-6 and tumor necrosis factor-α on the expression of angiogenic and collagenolytic factors in premature and mature adipocytes." in: Biochemical and biophysical research communications, Vol. 531, Issue 3, pp. 297-304, (2020) (PubMed).

    Kim, Yu, Yeo, Cho, Lee, Cho, Cho, Lee, Kim, Lee, Jun, Park, Yoon, Kim: "Prediction of Response to Sorafenib in Hepatocellular Carcinoma: A Putative Marker Panel by Multiple Reaction Monitoring-Mass Spectrometry (MRM-MS)." in: Molecular & cellular proteomics : MCP, Vol. 16, Issue 7, pp. 1312-1323, (2018) (PubMed).

    Retzlaff, Thamm, Ghosh, Ziegler, Haller, Parikh, David: "Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis." in: Scientific reports, Vol. 7, pp. 44113, (2017) (PubMed).

    Aktaş, Akbulut Yazici, Zengin, Akgün, Üstüner, Içli: "A new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin1 predicts survival in patients with advanced gastric cancer." in: Turkish journal of medical sciences, Vol. 47, Issue 2, pp. 399-406, (2017) (PubMed).

    Yang, Chen, Yang, Crane, Yang, Wang, Dong, Yi, Xie, Jing, Cai, Wang: "The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker." in: PeerJ, Vol. 5, pp. e3387, (2017) (PubMed).

    Yang, Chen, Yang, Crane, Huang, Dong, Yi, Guo, Cai, Wang: "Cervical cancer cell-derived angiopoietins promote tumor progression." in: Tumour biology, Vol. 39, Issue 7, pp. 1010428317711658, (2017) (PubMed).

    Gorzelak-Pabiś, Chalubinski, Wojdan, Luczak, Duraj, Mozdzan, Broncel: "Increased plasma concentrations of interleukin 35 in patients with coronary artery disease." in: Archives of medical science : AMS, Vol. 13, Issue 4, pp. 778-784, (2017) (PubMed).

  • 抗原 See all Angiopoietin 1 (ANGPT1) ELISA试剂盒
    Angiopoietin 1 (ANGPT1)
    别名
    ANGPT1 (ANGPT1 产品)
    别名
    AGP1 ELISA Kit, AGPT ELISA Kit, ANG1 ELISA Kit, 1110046O21Rik ELISA Kit, Ang-1 ELISA Kit, Ang1 ELISA Kit, Agpt ELISA Kit, Agpt1 ELISA Kit, ang1 ELISA Kit, wu:fc76g06 ELISA Kit, ANG-1 ELISA Kit, angpt1 ELISA Kit, MGC80788 ELISA Kit, angiopoietin 1 ELISA Kit, angiopoietin 1 S homeolog ELISA Kit, ANGPT1 ELISA Kit, Angpt1 ELISA Kit, angpt1 ELISA Kit, angpt1.S ELISA Kit
    UniProt
    Q15389
    途径
    RTK signaling, Glycosaminoglycan Metabolic Process
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