IL-18 ELISA 试剂盒
(8 references)-
- 抗原 See all IL-18 (IL18) ELISA试剂盒
- IL-18 (IL18) (Interleukin 18 (IL18))
- 抗原表位
- AA 33-197
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-18
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: Y33-D197 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <1pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: Y33-D197 - Featured
- Discover our best selling IL18 ELISA Kit
- Top Product
- Discover our top product IL18 ELISA Kit
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Interleukin 18 (IL18) ELISA Kit
IL18 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Interleukin 18 (IL18) ELISA KitIL18 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Interleukin 18 (IL18) ELISA KitIL18 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitHigh Sensitivity IL18 适用: 人 Colorimetric Sandwich ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 小鸡 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitSmall Sample IL18 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 马 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the IL-1 family.
- 板类型
- Pre-coated
- 实验流程
- human IL-18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18 has been precoated onto 96-well plates. Standards(E.coli, Y33-D197) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-18 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 135, Standard deviation: 5.8, CV(%): 4.3
- Sample 2: n=16, Mean(pg/ml): 380, Standard deviation: 23.2, CV(%): 6.1
- Sample 3: n=16, Mean(pg/ml): 762, Standard deviation: 41.9, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 141, Standard deviation: 7.3, CV(%): 5.2
- Sample 2: n=24, Mean(pg/ml): 425, Standard deviation: 29.3, CV(%): 6.9
- Sample 3: n=24, Mean(pg/ml): 776, Standard deviation: 45.1, CV(%): 5.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Pyroptosis induced by enterovirus 71 and coxsackievirus B3 infection affects viral replication and host response." in: Scientific reports, Vol. 8, Issue 1, pp. 2887, (2018) (PubMed).
: "miRNA-223-3p regulates NLRP3 to promote apoptosis and inhibit proliferation of hep3B cells." in: Experimental and therapeutic medicine, Vol. 15, Issue 3, pp. 2429-2435, (2018) (PubMed).
: "Regulatory B cells induced by pancreatic cancer cell-derived interleukin-18 promote immune tolerance via the PD-1/PD-L1 pathway." in: Oncotarget, Vol. 9, Issue 19, pp. 14803-14814, (2018) (PubMed).
: "The effect of salusin-β on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)." in: ARYA atherosclerosis, Vol. 14, Issue 1, pp. 1-10, (2018) (PubMed).
: "Interplay between Matrix Metalloproteinase-9, Matrix Metalloproteinase-2, and Interleukins in Multiple Sclerosis Patients." in: Disease markers, Vol. 2016, pp. 3672353, (2017) (PubMed).
: "Human cytomegalovirus triggers the assembly of AIM2 inflammasome in THP-1-derived macrophages." in: Journal of medical virology, (2017) (PubMed).
: "Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis." in: Oncogene, Vol. 36, Issue 48, pp. 6725-6737, (2017) (PubMed).
: "Management. The name game." in: Nursing times, Vol. 85, Issue 1, pp. 44-5, (1989) (PubMed).
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: "Pyroptosis induced by enterovirus 71 and coxsackievirus B3 infection affects viral replication and host response." in: Scientific reports, Vol. 8, Issue 1, pp. 2887, (2018) (PubMed).
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- 抗原 See all IL-18 (IL18) ELISA试剂盒
- IL-18 (IL18) (Interleukin 18 (IL18))
- 别名
- IL18 (IL18 产品)
- 背景
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Protein Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Background: Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3
Synonyms: Interleukin-18,IL-18,Iboctadekin,Interferon gamma-inducing factor,IFN-gamma-inducing factor,Interleukin-1 gamma,IL-1 gamma,IL18,IGIF, IL1F4,
Full Gene Name: Interleukin-18
Cellular Localisation: Secreted. - 基因ID
- 3606
- UniProt
- Q14116
- 途径
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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