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IL-18 ELISA 试剂盒

Name: IL-18 ELISA 试剂盒
SKU: ABIN411383
Availability: OutOfStock

This 大鼠 IL-18 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 IL-18. This ELISA Kit has been cited in 7+ publications.

ELISA

1 image

(7 references)

产品编号 ABIN411383

发货至: 中国

Datasheet as PDF

Quick Overview for IL-18 ELISA 试剂盒 (ABIN411383)

抗原

See all IL-18 (IL18) ELISA试剂盒

IL-18 (IL18) (Interleukin 18 (IL18))

抗原表位

AA 37-194

适用

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Discover our top product IL18 ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-18

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: H37-S194

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<1pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: H37-S194
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Interleukin 18 (IL18) ELISA Kit
IL18 适用: 小鸡 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

rat IL-18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18 has been precoated onto 96-well plates. Standards(E.coli, H37-S194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-18 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat IL-18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IL-18 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 126, Standard deviation: 6, CV(%): 4.7
- Sample 2: n=16, Mean(pg/ml): 335, Standard deviation: 19.43, CV(%): 5.8
- Sample 3: n=16, Mean(pg/ml): 592, Standard deviation: 36.7, CV(%): 6.2,
- Sample 1: n=24, Mean(pg/ml): 147, Standard deviation: 7.94, CV(%): 5.4
- Sample 2: n=24, Mean(pg/ml): 392, Standard deviation: 24.7, CV(%): 6.3
- Sample 3: n=24, Mean(pg/ml): 644, Standard deviation: 46.4, CV(%): 7.2
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Fu, Wang, Cai, Zhao, Fu: "Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats." in: Drug design, development and therapy, Vol. 12, pp. 1609-1619, (2018) (PubMed).

Cao, Fang, Lu, Qian, Ma, He, Pi, Yu, Zhou: "Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats." in: International journal of nanomedicine, Vol. 11, pp. 3331-46, (2017) (PubMed).

Dong, Zhong, Chen, Wu, Zhang, Huang, Yang: "Effect of Acupuncture on Endometrial Angiogenesis and Uterus Dendritic Cells in COH Rats during Peri-Implantation Period." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3647080, (2017) (PubMed).

Tian, Zhu, Yao, Meng, Wang, Xie, Wang: "NLRP3 participates in the regulation of EMT in bleomycin-induced pulmonary fibrosis." in: Experimental cell research, Vol. 357, Issue 2, pp. 328-334, (2017) (PubMed).

Li, Hu, Wang, Zhang, Zhou, Yang, Li, Xiong, Liu, Li, Wu, Zheng: "Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis." in: Oncogene, Vol. 36, Issue 48, pp. 6725-6737, (2017) (PubMed).

Meng, Gong, Fang, Song, Wu, Zhou, Qian: "Effect of interferon-γ on NF-κB and cytokine IL-18 and IL-27 in acute pancreatitis." in: Bosnian journal of basic medical sciences, Vol. 13, Issue 2, pp. 114-8, (2014) (PubMed).

Yang, Shen, Chen, Ge: "Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion." in: World journal of gastroenterology, Vol. 11, Issue 29, pp. 4524-9, (2005) (PubMed).
抗原 See all IL-18 (IL18) ELISA试剂盒

IL-18 (IL18) (Interleukin 18 (IL18))

别名

IL18

背景

Protein Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Background: Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3
Synonyms: Interleukin-18,IL-18,Interferon gamma-inducing factor,IFN-gamma-inducing factor,Interleukin-1 gamma,IL-1 gamma,Il18,Igif,
Full Gene Name: Interleukin-18
Cellular Localisation: Secreted.

基因ID

29197

UniProt

P97636

途径

Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome