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VEGFC ELISA 试剂盒

Name: VEGFC ELISA 试剂盒
SKU: ABIN411382
Availability: OutOfStock

人 VEGFC ELISA Kit, Colorimetric is an assay for quantification of 人 VEGFC, that has been mentioned in 10+ publications.

ELISA

1 image

(10 references)

产品编号 ABIN411382

发货至: 中国

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Quick Overview for VEGFC ELISA 试剂盒 (ABIN411382)

抗原

See all VEGFC ELISA试剂盒

VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

抗原表位

AA 103-227

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Discover our top product VEGFC ELISA Kit

最低检测浓度

62.5 pg/mL

原理

Sandwich Human VEGF-C ELISA Kit to quantitate Human VEGFC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method

Quantitative

特异性

Expression system for standard: NS0
Immunogen sequence: T103-R227
Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 3 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit
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Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit
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Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit
VEGFC 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit
VEGFC 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit
VEGFC 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit
Small Sample VEGFC 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit
VEGFC 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit
VEGFC 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit
VEGFC 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit
VEGFC 适用: 小鼠 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Plasma, Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 4.7%, 6.7%, 5.4%
Inter-Assay Precision (CV%): 6.2%, 8.6%, 5.7%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Dai, Cui, Zhang, Cheng, Liu, Yang, Fan, Wang, Lin, Zhang, Li, Mao, Wang, Su, Zhang, Peng, Yang, Hu, Yang, Huang, Xiang, Yu, Zhou, Wei, Deng: "SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin." in: Nature communications, Vol. 7, pp. 11996, (2018) (PubMed).

Zhu, Huang, Huang, Zheng, Hua, Yang, Zhuang, Wang, Ye: "Lipopolysaccharide increases the release of VEGF-C that enhances cell motility and promotes lymphangiogenesis and lymphatic metastasis through the TLR4- NF-κB/JNK pathways in colorectal cancer." in: Oncotarget, Vol. 7, Issue 45, pp. 73711-73724, (2018) (PubMed).

Xu, Ge, Han, Yang, Su, Zhao, Zhao, Yang, Yang: "High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma." in: World journal of gastroenterology : WJG, Vol. 21, Issue 11, pp. 3256-65, (2015) (PubMed).

Li, Wang, Zhao, Yang, Li, Zhao, Yu, Wang, Meng, Liu, Zhu, Gao, Jia, Yang: "Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis." in: Breast cancer research : BCR, Vol. 16, Issue 5, pp. 454, (2015) (PubMed).

Song, Yue, Li, Li, Zhao, Zhang: "Study of the mechanism of sonodynamic therapy in a rat glioma model." in: OncoTargets and therapy, Vol. 7, pp. 1801-10, (2014) (PubMed).

Zhang, Ding, Diao, Yan, Sun, Hu: "CYR61 modulates the vascular endothelial growth factor C expression of decidual NK cells via PI3K/AKT pathway." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 3, pp. 216-23, (2012) (PubMed).

Chuangui, Peng, Zhentao: "The expression of high mobility group box 1 is associated with lymph node metastasis and poor prognosis in esophageal squamous cell carcinoma." in: Pathology oncology research : POR, Vol. 18, Issue 4, pp. 1021-7, (2012) (PubMed).

Pan, Ren, Ma, Liu, Yu, Ji, Pan, Li, Yang, Lv, Shen, Chen, Zhang, Willard, He, Zheng: "High-density lipoprotein of patients with type 2 diabetes mellitus elevates the capability of promoting migration and invasion of breast cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 1, pp. 70-82, (2012) (PubMed).

Yang, Shi, Li, Yi: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." in: Oncology reports, Vol. 25, Issue 1, pp. 259-65, (2010) (PubMed).

Wang, Zhao, Guo, Zhang, Xie, Xiang, Gao, Wang, Chen: "The expression of RNA-binding protein HuR in non-small cell lung cancer correlates with vascular endothelial growth factor-C expression and lymph node metastasis." in: Oncology, Vol. 76, Issue 6, pp. 420-9, (2009) (PubMed).
抗原 See all VEGFC ELISA试剂盒

VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

别名

VEGFC

背景

Background: Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D (VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein (BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23Kda after glycosylation.
Gene Full Name: vascular endothelial growth factor C

基因ID

7424

UniProt

P49767

途径

RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2