Growth Hormone 1 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for Growth Hormone 1 ELISA 试剂盒 (ABIN411378)
抗原
See all Growth Hormone 1 (GH1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 15.6 pg/mL
-
原理
- Sandwich Human Growth Hormone/GH1 ELISA Kit to quantitate Human GH1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: E.coli
Immunogen sequence: F27-F217Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 2 pg/mL
-
组件
-
- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Growth Hormone 1 (GH1) ELISA Kit
GH1 适用: 犬 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
-
实验精密度
-
Intra-Assay Precision (CV%): 7.6%, 5.8%, 7.7%
Inter-Assay Precision (CV%): 8.2%, 6.3%, 9.4% -
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
-
有效期
- 12 months
-
-
- Growth Hormone 1 (GH1)
-
别名
- GH1
-
背景
-
Background: Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.
Gene Full Name: growth hormone 1
-
基因ID
- 2688
-
UniProt
- P01241
-
途径
- NF-kappaB Signaling, JAK/STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process
抗原 See all Growth Hormone 1 (GH1) ELISA试剂盒
-
