FLT1 ELISA 试剂盒
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Quick Overview for FLT1 ELISA 试剂盒 (ABIN411372)
抗原
See all FLT1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human VEGFR1/FLT1 ELISA Kit to quantitate Human FLT1 in cell culture supernatants, serum and plasma (EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: S27-H687Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 30 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA Kit
FLT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: 人 Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: 人 Colorimetric Sandwich ELISA 1.5-400 ng/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Follicular Fluid, Plasma, Saliva, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) ELISA KitFLT1 适用: 小鼠 AA 23-759 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.1%, 7.8%, 4.6%
Inter-Assay Precision (CV%): 4.7%, 9.7%, 4.7% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene." in: Annals of dermatology, Vol. 28, Issue 1, pp. 30-9, (2016) (PubMed).
: "Evaluation of the relationship between serum levels of VEGF and sVEGFR1 with mortality and prognosis in patients with Crimean-Congo hemorrhagic fever." in: Journal of medical virology, Vol. 85, Issue 10, pp. 1794-801, (2013) (PubMed).
: "Soluble Fms-like tyrosine kinase-1 expression inhibits the growth of multiple myeloma in nude mice." in: Acta biochimica et biophysica Sinica, Vol. 39, Issue 7, pp. 499-506, (2007) (PubMed).
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- FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
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别名
- FLT1
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背景
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Background: Vascular endothelial growth factor receptor 1 is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.
Gene Full Name: fms related receptor tyrosine kinase 1
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基因ID
- 2321
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UniProt
- P17948
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途径
- RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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